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duxiu/initial_release/微观组织的分析电子显微学表征英文版_13153334.zip

微观组织的分析电子显微学表征英文版 YonghuaRong;戎咏华著, Rong Yong Hua, Yonghua Rong 北京:高等教育出版社, 2012, 2012

'Characterization of Microstructures by Analytical Electron Microscopy (AEM)' describes the basic concepts and operative techniques of AEM. It focuses on the study of phase transformations and dislocation in deformation by AEM. Further, the book also presents the physical concepts and mathematic analysis for diffraction and crystallography using numerous examples, such as the quantitative prediction of the orientation relationships in phase transformations. The book is intended for researchers and graduate students in materials science and engineering, and condensed matter physics. Yonghua Rong is a professor at School of Materials Science and Engineering, Shanghai Jiao Tong University, China

英语 [en] · 中文 [zh] · PDF · 75.3MB · 2012 · 📗 未知类型的图书 · 🚀/duxiu/zlibzh · Save

ia/correlativelight0000unse.pdf

Correlative Light and Electron MIcroscopy (Volume 111) (Methods in Cell Biology, Volume 111) Thomas Mueller-Reichert, Paul Verkade Amsterdam, Netherlands ; Boston, Mass.: Elsevier / Academic Press, Elsevier Ltd., Amsterdam, Netherlands, 2012

xix, 404 p., [40] p. of plates : 24 cm The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field. Includes a brief history and overview on CLEM methods Includes bibliographical references and index

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scihub/10.1007/978-1-59259-232-6.pdf

Methods in Plant Electron Microscopy and Cytochemistry || William V. Dashek (auth.), William V. Dashek (eds.) Humana Press : Imprint : Humana Press, 10.1007/97, 2000

Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.

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ia/electromicroscop00mana.pdf

Electron Microscopy Methods And Protocols (methods In Molecular Biology) Hajibagheri, M. A. Nasser Humana Press; Brand: Humana Press, Springer Nature, Totowa, N.J., 1999

In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.

英语 [en] · PDF · 36.2MB · 1999 · 📗 未知类型的图书 · 🚀/ia · Save

nexusstc/Electron Microscopy XIV: Selected, peer reviewed papers from the XIV International Conference on Electron Microscopy (EM2011), June 26-30, 2011, Wisla, Poland/1bbfd709af70612a444212ae7fe21181.pdf

Electron Microscopy XIV: Selected, Peer Reviewed Papers from the XIV International Conference on Electron Microscopy Em2011, June 26-30, 2011, Wisla, Poland (Solid State Phenomena) edited by Danuta Stróż and Krystian Prusik Trans Tech Publications Ltd., Trans Tech Publications, Ltd, Durnten-Zürich, 2012

These are the proceedings of the XIV International Conference on Electron Microscopy (EM2011) held in Wisła, Poland from the 26 to 30th June 2011. The goal of the conference was to provide a forum where researchers from many different countries could exchange their latest advances in the field of structural studies, regarding the use of electron microscopic techniques as applied to various materials. Plenary and invited lectures offered overviews of exciting new developments which highlighted the applications of new electron microscopic techniques in physics, chemistry, materials science and in life and earth sciences. Volume is indexed by Thomson Reuters CPCI-S (WoS).The papers cover topics such as electron, holography, tomography, HREM, STEM, EBSD, ED and precession techniques - as well as their application to materials science and related disciplines. Researchers from different countries exchange recent experiences in structural studies that use electron microscopy techniques on many different materials. Among 77 papers are discussions of the art and application of large angle convergent beam electron diffraction, near grain boundary behavior of aluminum bicrystals deformed in plane strain conditions, the structure and morphology of thin films deposited by pulsed laser deposition techniques, the positive hydrogen effect in structure surface layers on titanium alloys, and microstructural changes induced during the hydrogen charging process in stainless steels with and without nitrided layers

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ia/cellularelectron0079unse.pdf

Cellular Electron Microscopy (Volume 79) (Methods in Cell Biology, Volume 79) edited by J. Richard McIntosh Amsterdam: Boston: Elsevier/Academic Press, Elsevier Ltd., Amsterdam, 2007

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. Updates the numerous technological innovations that have improved the capabilities of electron microscopy Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging Chapters include a balance of'how to','so what'and'where next', providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

英语 [en] · PDF · 59.5MB · 2007 · 📗 未知类型的图书 · 🚀/ia · Save

ia/cellularelectron00jric.pdf

Cellular Electron Microscopy (Volume 79) (Methods in Cell Biology, Volume 79) edited by J. Richard McIntosh Amsterdam: Boston: Elsevier/Academic Press, Elsevier Ltd., Amsterdam, 2007

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. Updates the numerous technological innovations that have improved the capabilities of electron microscopy Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging Chapters include a balance of'how to','so what'and'where next', providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

英语 [en] · PDF · 49.0MB · 2007 · 📗 未知类型的图书 · 🚀/ia · Save

nexusstc/Nanocharacterisation/01be26a57087ac1d6208e56056227280.pdf

Nanocharacterisation (Nanoscience, Volume 37) Angus I. Kirkland, Sarah J. Haigh, Paul O'Brien, David J. Smith, S. J. Pennycook, Rafal Dunin-Borkowski, Martin Castell, D. Bell The Royal Society of Chemistry, Nanoscience & Nanotechnology Series, RSC nanoscience & nanotechnology, 2, 2015

Nanocharacterisation provides an overview of the main characterisation techniques that are currently used to study nanostructured materials. Following on from the success of the first edition, this new edition has been fully revised and updated to reflect the recent developments in instrumental characterisation methods. With contributions from internationally recognised experts, each chapter focuses on a different technique to characterise nanomaterials providing experimental procedures and applications. State of the art characterisation methods covered include Transmission Electron Microscopy, Scanning Transmission Electron Microscopy, Scanning Probe Microscopy, Electron Energy Loss Spectroscopy and Energy Dispersive X-ray Analysis, 3D Characterisation, Scanning Electron and Ion Microscopy and In situ Microscopy. Essentially a handbook to all working in the field this indispensable resource will appeal to academics, professionals and anyone working fields related to the research and development of nanocharacterisation and nanotechnology.

英语 [en] · PDF · 25.9MB · 2015 · 📘 非小说类图书 · 🚀/duxiu/lgli/lgrs/nexusstc/zlib · Save

lgli/MMB/MMB vol.369 Kuo J. (ed.) Electron Microscopy Methods and Protocols (Humana Press, 2007)(ISBN 1588295737)(625s).pdf

Methods in Molecular Biology, Volume 369: Electron Microscopy: Methods and Protocols John J. Bozzola (auth.), John Kuo (eds.) Humana Press, Methods in molecular biology -- 369, Methods in molecular biology (Clifton, N.J.) -- 369, 2nd ed. / edited by John Kuo, Totowa, N.J, New Jersey, 2007

The revised and expanded second edition of this book presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

英语 [en] · PDF · 14.1MB · 2007 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/scihub/zlib · Save

nexusstc/Electron Microscopy/c70f96816e3ded972342655a7991e3c1.pdf

Methods in Molecular Biology, Volume 369: Electron Microscopy: Methods and Protocols John J. Bozzola (auth.), John Kuo (eds.) Humana Press, Methods in molecular biology -- 369, Methods in molecular biology (Clifton, N.J.) -- 369, 2nd ed. / edited by John Kuo, Totowa, N.J, New Jersey, 2007

The revised and expanded second edition of this book presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

英语 [en] · PDF · 17.2MB · 2007 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/scihub/zlib · Save

duxiu/initial_release/OPTICAL TECHNIQUES IN BIOLOGCAL RESEARCH_40104677.zip

Optical Techniques in Biological Research (Physical Techniques in Biology and Medicine Series) DENIS L. ROUSSEAU, edited by Denis L. Rousseau, Rousseau, Denis Academic Press, Incorporated, 1984, 1984

Optical Techniques in Biological Research discusses the fundamentals and applications of light scattering, Raman scattering, Fourier transform infrared spectroscopy, nanosecond fluorescence spectroscopy, and circular dichroism. Electron microscopy is also included owing to some of its classical similarity to optical microscopy optical structural and resonance techniques for biological research. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Structural and Resonance Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.

英语 [en] · PDF · 22.9MB · 1984 · 📗 未知类型的图书 · 🚀/duxiu/zlibzh · Save

upload/newsarch_ebooks_2025_10/2019/02/05/111997111X.pdf

Low Voltage Electron Microscopy : Principles and Applications Bell, David C. (editor);Erdman, Natasha (editor) John Wiley & Sons, Ltd, 2012 dec 17

Part of the Wiley-Royal Microscopical Society Series , this book discusses the rapidly developing cutting-edge field of low-voltage microscopy, a field that has only recently emerged due to the rapid developments in the electron optics design and image processing. It serves as a guide for current and new microscopists and materials scientists who are active in the field of nanotechnology, and presents applications in nanotechnology and research of surface-related phenomena, allowing researches to observe materials as never before. Content: Chapter 1 Introduction to the Theory and Advantages of Low Voltage Electron Microscopy (pages 1–30): David C. Bell and Natasha Erdman Chapter 2 SEM Instrumentation Developments for Low kV Imaging and Microanalysis (pages 31–55): Natasha Erdman and David C. Bell Chapter 3 Extreme High?Resolution (XHR) SEM Using a Beam Monochromator (pages 57–71): Richard J. Young, Gerard N. A. van Veen, Alexander Henstra and Lubomir Tuma Chapter 4 The Application of Low?Voltage SEM—From Nanotechnology to Biological Research (pages 73–95): Natasha Erdman and David C. Bell Chapter 5 Low Voltage High?Resolution Transmission Electron Microscopy (pages 97–117): David C. Bell Chapter 6 Gentle STEM of Single Atoms: Low keV Imaging and Analysis at Ultimate Detection Limits (pages 119–161): Ondrej L. Krivanek, Wu Zhou, Matthew F. Chisholm, Juan Carlos Idrobo, Tracy C. Lovejoy, Quentin M. Ramasse and Niklas Dellby Chapter 7 Low Voltage Scanning Transmission Electron Microscopy of Oxide Interfaces (pages 163–184): Robert Klie Chapter 8 What's Next? The Future Directions in Low Voltage Electron Microscopy (pages 185–200): David C. Bell and Natasha Erdman

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duxiu/initial_release/ELECTRON BEAM ANALYSIS OF MATERIALS_40564916.zip

ELECTRON BEAM ANALYSIS OF MATERIALS M. H. Loretto CHAPMAN AND HALL LTD, 1984, 1984

Introduction To Electron Beam Instruments -- Electron-specimen Interactions -- Layout And Operational Modes Of Electron Beam Instruments -- Interpretation Of Diffraction Information -- Analysis Of Micrographs In Tem, Stem, Hrem, And Sem -- Interpretation Of Analytical Data. M.h. Loretto. Includes Bibliographies And Index. The second edition of Electron Beam Analysis of Materials provides a concise and up-to-date overview of the most widely used electron beam instruments and techniques of microstructural analysis available today.

英语 [en] · PDF · 57.9MB · 1984 · 📗 未知类型的图书 · 🚀/duxiu/zlibzh · Save

lgli/A:\_for_add\1\SD\9780123339263 (13).pdf

Basic techniques for transmission electron microscopy mit Tab M. Hayat (Auth.) Elsevier Science & Technology Books, Elsevier Ltd., Orlando, 1986

Basic Techniques For Transmission Electron Microscopy Abstract: Basic Techniques For Transmission Electron Microscopy

英语 [en] · PDF · 12.4MB · 1986 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

nexusstc/_\_In-Situ\_\_ Electron Microscopy at High Resolution/93832a0aca618761730d6563079f372e.pdf

\_\_In-Situ\_\_ Electron Microscopy at High Resolution Florian Banhart; World Scientific (Firm) World Scientific Publishing Company, World Scientific Publishing Company, Singapore, 2008

In-situ high-resolution electron microscopy is a modern and powerful technique in materials research, physics, and chemistry, but no book covering it has been published until now. In-situ techniques are not even treated in textbooks of electron microscopy. Thus, there is a need to collect the present knowledge about the techniques and achievements of in-situ electron microscopy in one book. Since high-resolution electron microscopes are available in most modern laboratories of materials science, more and more scientists or students are starting to work on this subject. In this comprehensive volume, the most important techniques and achievements of in-situ high-resolution electron microscopy will be reviewed by renowned experts. Applications in several fields of materials science will also be demonstrated.

英语 [en] · PDF · 10.9MB · 2008 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

nexusstc/Functional ultrastructure./a671d6afe7adfb6246febbdcac982cf6.pdf

Functional ultrastructure : an atlas of tissue biology and pathology Margit Pavelka, Jürgen Roth. Springer Verlag Wien,, Springer Nature, New York, 2005

This atlas of functional ultrastructure provides not only a detailed insight into the complex structure and organization of cells and tissues but also into specific functions fulfilled by the various cellular organelles and the dynamics of the different processes inside cells. The large collection of electron micrographs, together with those from immunoelectron microscopy, is complemented by thorough explanatory texts and schemes. Emphasis was placed on an integrated view of structure and function to show that subcellular organelles provide the structural foundation for fundamental processes of living organisms. Specialized cell types form the various tissues, and principles of tissue organization are presented. Under various conditions of disease, characteristic structural alterations may occur which are illustrated with examples thus providing a valuable source of information for scientists and students of Medicine and Biological Sciences, particularly of Histology, Cell and Molecular Biology.

英语 [en] · PDF · 127.6MB · 2005 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

zlib/Biology and other natural sciences/Cytology/Pavelka, Margit.; Roth, Jürgen./Functional Ultrastructure : An Atlas of Tissue Biology and Pathology_119702099.pdf

Functional Ultrastructure : An Atlas of Tissue Biology and Pathology Pavelka, Margit.; Roth, Jürgen. Springer Verlag Wien,, Springer Nature, New York, 2005

The period between 1950 and 1980 were the golden It also provides unique insights into how pathological years of transmission electron microscopy and pro- processes affect cell organization. duced a plethora of new information on the structure of This information is vital to current work in which cells that was coupled to and followed by biochemical the emphasis is on integrating approaches from p- and functional studies. TEM was king and each micro- teomics, molecular biology, molecular imaging and graph of a new object produced new information that physiology, and pathology to understand cell functions led to new insights on cell and tissue organization and and derangements in disease. In this current era, there is their functions. The quality of data represented by the a growing tendency to substitute modern light mic- images of cells and tissues had been perfected to a very scopic techniques for electron microscopy because it is high level by the great microscopists of the era including less technically demanding and is more readily available Palade, Porter, Fawcett, Sjostrand, Rhodin and many to researchers. This atlas reminds us that the infor- others. At present, the images that we see in leading tion obtained by electron microscopy is invaluable and journals for the most part do not reach the same techni- has no substitute.

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scihub/10.1007/978-1-4939-0935-3.pdf

Surface Microscopy with Low Energy Electrons || Ernst Bauer (auth.) Springer New York : Imprint: Springer, 10.1007/97, 2014

This book, written by a pioneer in surface physics and thin film research and the inventor of Low Energy Electron Microscopy (LEEM), Spin-Polarized Low Energy Electron Microscopy (SPLEEM) and Spectroscopic Photo Emission and Low Energy Electron Microscopy (SPELEEM), covers these and other techniques for the imaging of surfaces with low energy (slow) electrons. These techniques also include Photoemission Electron Microscopy (PEEM), X-ray Photoemission Electron Microscopy (XPEEM), and their combination with microdiffraction and microspectroscopy, all of which use cathode lenses and slow electrons. Of particular interest are the fundamentals and applications of LEEM, PEEM, and XPEEM because of their widespread use. Numerous illustrations illuminate the fundamental aspects of the electron optics, the experimental setup, and particularly the application results with these instruments. Surface Microscopy with Low Energy Electrons will give the reader a unified picture of the imaging, diffraction, and spectroscopy methods that are possible using low energy electron microscopes. Provides a unified description of full-field, low energy electron microscopies Presents the basic theory and experiment of low energy emission and reflection Compares the possibilities and limitations of the various imaging methods Describes multi-method studies Contains an extensive list of references for easy access to the original literature /ul>

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upload/newsarch_ebooks_2025_10/2022/05/29/Physical Principles of Electron Microscopy An Introduction .pdf

Physical Principles of Electron Microscopy : An Introduction to TEM, SEM, and AEM R.F. Egerton (auth.) Springer International Publishing : Imprint : Springer, 2nd ed. 2016, Cham, 2016

This popular textbook provides an introduction to the theory and practice of electron microscopy. The second edition has been updated to reflect the recent developments, including correction of lens aberrations in a TEM column and new material on environmental TEM and SEM. The text is linked to a new website that contains additional educational material such as sample exam questions and answers to selected problems. This edition also contains expanded reference lists that allow the reader to efficiently explore key topics in greater depth.Scanning and fixed-beam electron microscopes are an indispensable tool for both research and routine evaluation in the physical, biological and medical sciences, including specialized fields in materials science, nanotechnology and semiconductor processing. __Physical Principles of Electron Microscopy, Second Edition__, is ideal for students, researchers, and technologists who make use of electron microscopes but have only a limited knowledge of physics and mathematics. Undergraduate students will understand how basic principles of physics are utilized in this important area of applied science, while university teachers and researchers will find a concise but authoritative teaching, supplemental, or reference text covering the basic principles and practice of microscopy.

英语 [en] · PDF · 7.3MB · 2016 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/scihub/upload/zlib · Save

ia/physicalprincipl0000eger.pdf

Physical Principles of Electron Microscopy : An Introduction to TEM, SEM, and AEM Ray F. Egerton Springer Science+Business Media, Inc, Springer Nature, Boston, MA, 2005

Scanning and stationary-beam electron microscopes have become an indespensible tool for both research and routine evaluation in materials science, the semiconductor industry, nanotechnology and the biological, forensic, and medical sciences. This book provides an introduction to the theory and current practice of electron microscopy, aimed primarily at undergraduates who need to learn how the basic principles of physics are applied in an important area of science and technology that has contributed greatly to our knowledge of life processes and'inner space.'However, it will be equally valuable for technologists who make use of electron microscopes and for graduate students, university teachers and researchers who need a concise text that deals with the basic principles of microscopy. Less technical but broader in scope than other microscopy textbooks, Physical Principles of Electron Microscopy is appropriate for undergraduates and technologists with limited mathematical training.

英语 [en] · PDF · 12.0MB · 2005 · 📗 未知类型的图书 · 🚀/ia · Save

lgli/D:\!genesis\library.nu\00\_139904.004aace83fa3c60e8d7d6afff44a6ad2.pdf

Three-dimensional electron microscopy of macromolecular assemblies $c Joachim Frank Academic Press, Incorporated, 1, FR, 1996

Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized* Describes methods that have been instrumental in exploring the three-dimensional structure of* the nuclear pore complex* the calcium release channel;* the ribosome* chaperonins

英语 [en] · PDF · 18.9MB · 1996 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

lgli/D:\!genesis\library.nu\11\_195123.11e5b8f4f0dc043a8ccf23eaa553f663.pdf

Hair Follicle : Differentiation Under the Electron Microscope - An Atlas Kiyokazu Morioka Springer-Verlag Tokyo, 1 edition, December 15, 2004

"The electron microscope has been used in hair research for half a century, but the knowledge gained through this study has become rather fragmented over the years. While the molecular biological study of hair has become an active field, even here, as in more traditional research, an understanding of the morphological aspects is essential because the hair follicle structure is highly complex. The author explicates the structure, development, and differentiation of the hair follicle, using his own original photographs and explanations of current research, including growth factors, differentiation-inducers, cell signaling pathways, and transcription factors. With neonatal rats as subjects, he focuses on the morphological analysis of all basic hair cells - in the shaft, matrix, and papilla - from birth to death. The introductory chapter will benefit novices, with the following sections consisting mainly of explanations of the electron microscopic photographs of hair cells."--BOOK JACKET

英语 [en] · PDF · 18.2MB · 2004 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/scihub/zlib · Save

upload/newsarch_ebooks_2025_10/2018/02/06/Hair Follicle Differentiation under the Electron Microscope.pdf

Hair Follicle : Differentiation Under the Electron Microscope - An Atlas Kiyokazu Morioka Springer-Verlag Tokyo, 1 edition, December 15, 2004

"The electron microscope has been used in hair research for half a century, but the knowledge gained through this study has become rather fragmented over the years. While the molecular biological study of hair has become an active field, even here, as in more traditional research, an understanding of the morphological aspects is essential because the hair follicle structure is highly complex. The author explicates the structure, development, and differentiation of the hair follicle, using his own original photographs and explanations of current research, including growth factors, differentiation-inducers, cell signaling pathways, and transcription factors. With neonatal rats as subjects, he focuses on the morphological analysis of all basic hair cells - in the shaft, matrix, and papilla - from birth to death. The introductory chapter will benefit novices, with the following sections consisting mainly of explanations of the electron microscopic photographs of hair cells."--BOOK JACKET

英语 [en] · PDF · 18.1MB · 2004 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/upload/zlib · Save

lgli/Cs_Computer science/CsIp_Image processing/Frank J. Three-dimensional electron microscopy of macromolecular assemblies.. Visualization of biological molecules (2ed., OUP, 2006)(ISBN 0195150961)(428s)_CsIp_.pdf

Three-Dimensional Electron Microscopy of Macromolecular Assemblies : Visualization of Biological Molecules in Their Native State by Joachim Frank Oxford University Press, USA, 2nd ed, Oxford ; New York, 2006

Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are. <h3>Booknews</h3> For new users of electron microscopes for investigating molecular-scale structures, a systematic introduction to the single-particle methods of reconstruction, focusing on random- conical reconstructions as developed at the State University of New York in Albany. Among the techniques are two-dimensional averaging, multivariate statistical analysis and classification of images, three-dimensional reconstructions, and interpreting images. The calcium release channel is described as an example of applications. An appendix discusses software implementations. Annotation c. Book News, Inc., Portland, OR (booknews.com)

英语 [en] · PDF · 9.0MB · 2006 · 📘 非小说类图书 · 🚀/duxiu/lgli/lgrs/nexusstc/zlib · Save

nexusstc/Cryo-EM Part A: Sample Preparation and Data Collection/d639651b067b2ffeedf3be8ad9f064b9.pdf

Cryo-EM Part A: Sample Preparation and Data Collection (Volume 481) (Methods in Enzymology, Volume 481) edited by Grant J. Jensen Academic Press/Elsevier, Methods in Enzymology, Methods in Enzymology 481, 1, 2010

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.\* Includes time-tested core methods and new innovations applicable to any researcher \* Methods included are useful to both established researchers and newcomers to the field \* Relevant background and reference information given for procedures can be used as a guide

英语 [en] · PDF · 32.0MB · 2010 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

lgli/D:\!genesis\library.nu\2c\_11355.2c023f8594fa2dae2ac5b58292984e9f.pdf

Characterization of High Tc Materials and Devices by Electron Microscopy edited by Nigel D. Browning, Stephen J. Pennycook Cambridge University Press (Virtual Publishing), 1, 2000

This is a clear and up-to-date account of the application of electron-based microscopies to the study of high-Tc superconductors. Written by leading experts, this compilation provides a comprehensive review of scanning electron microscopy, transmission electron microscopy and scanning transmission electron microscopy, together with details of each technique and its applications. Introductory chapters cover the basics of high-resolution transmission electron microscopy, including a chapter devoted to specimen preparation techniques and microanalysis by scanning transmission electron microscopy. Ensuing chapters examine identification of new superconducting compounds, imaging of superconducting properties by low-temperature scanning electron microscopy, imaging of vortices by electron holography and electronic structure determination by electron energy loss spectroscopy. The use of scanning tunneling microscopy for exploring surface morphology, growth processes and the mapping of superconducting carrier distributions is also discussed. Final chapters consider applications of electron microscopy to the analysis of grain boundaries, thin films and device structures. Detailed references are included. This book will interest graduate students and researchers in condensed matter physics and material science.

英语 [en] · PDF · 14.1MB · 2000 · 📘 非小说类图书 · 🚀/duxiu/lgli/lgrs/nexusstc/zlib · Save

lgli/A:\usenetabtechnical\The Characterization of High Tc Mtls and Devs by Electron Microscopy - N. Browning, et al., (Cambridge, 2000) WW.pdf

Characterization of high Tc materials and devices by electron microscopy edited by Nigel D. Browning, Stephen J. Pennycook Cambridge University Press (Virtual Publishing), 1, 2000

This is a clear and up-to-date account of the application of electron-based microscopies to the study of high-Tc superconductors. Written by leading experts, this compilation provides a comprehensive review of scanning electron microscopy, transmission electron microscopy and scanning transmission electron microscopy, together with details of each technique and its applications. Introductory chapters cover the basics of high-resolution transmission electron microscopy, including a chapter devoted to specimen preparation techniques and microanalysis by scanning transmission electron microscopy. Ensuing chapters examine identification of new superconducting compounds, imaging of superconducting properties by low-temperature scanning electron microscopy, imaging of vortices by electron holography and electronic structure determination by electron energy loss spectroscopy. The use of scanning tunneling microscopy for exploring surface morphology, growth processes and the mapping of superconducting carrier distributions is also discussed. Final chapters consider applications of electron microscopy to the analysis of grain boundaries, thin films and device structures. Detailed references are included. This book will interest graduate students and researchers in condensed matter physics and material science.

英语 [en] · PDF · 36.2MB · 2000 · 📘 非小说类图书 · 🚀/duxiu/lgli/lgrs/nexusstc/zlib · Save

lgli/A:\_for_add\1\SD\series\0091679X-111 (26).pdf

Correlative Light and Electron MIcroscopy (Volume 111) (Methods in Cell Biology, Volume 111) Thomas Müller-Reichert and Paul Verkade (Eds.) Academic Press, Elsevier, Methods in Cell Biology, Methods in Cell Biology 111, 2012

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods

英语 [en] · PDF · 45.7MB · 2012 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

lgli/K:\_add\2\SD\0091679X-124.pdf

METHODS IN CELL BIOLOGY CORRELATIVE LIGHT AND ELECTRON MICROSCOPY II VOLUME 124 Thomas Mueller-Reichert, Paul Verkade Academic Press,Elsevier, Methods in Cell Biology Volume 124, 1. ed, 2014

This new volume of __Methods in Cell Biology__ looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.* Covers sections on model systems and functional studies, imaging-based approaches and emerging studies * Chapters are written by experts in the field * Cutting-edge material * Second of two volumes dedicated to Correlative Light and Electron microscopy (CLEM)

英语 [en] · PDF · 55.5MB · 2014 · 📘 非小说类图书 · 🚀/duxiu/lgli/lgrs/nexusstc/zlib · Save

ia/electronmicrosco0000unse_w2t1.pdf

Electron microscopy of plant cells. Ed. by J. L. Hall, C. Hawes John Lloyd Hall; Chris Hawes London ; San Diego, CA: Academic Press, Elsevier Ltd., London, 1991

Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

英语 [en] · PDF · 28.4MB · 1991 · 📗 未知类型的图书 · 🚀/ia · Save

nexusstc/Characterisation of displacement cascade damage in Cu/sub 3/Au produced by fusion-neutron irradiation/18ccc350c9e3e05454addb1db7fe89be.pdf

Characterisation of displacement cascade damage in Cu/sub 3/Au produced by fusion-neutron irradiation C A English Affiliation: UKAEA Atomic Energy Research Establishment, Harwell. Metallurgy Div.; M L Jenkins; M A Kirk Affiliation: Oxford Univ. (UK). Dept. of Metallurgy and Science of Materials Institute of Physiscs Publishing, CRC Press (Unlimited), Bristol [England], 2001

As the selection of material for particular engineering properties becomes increasingly important in keeping costs down, methods for evaluating material properties also become more relevant. One such method examines the geometry of grain boundaries, which reveals much about the properties of the material.Studying material properties from their geometrical measurements, The Measurement of Grain Boundary Geometry provides a framework for a specialized application of electron microscopy for metals and alloys and, by extension, for ceramics, minerals, and semiconductors. The book presents an overview of the developments in the theory of grain boundary geometry and its practical applications in material engineering. It also covers the tunneling electron microscope (TEM), experimental aspects of data collection, data processing, and examples from actual investigations. Each step of the analysis process is clearly described, from data collection through processing, analysis, representation, and display to applications. The book also includes a glossary of terms.Exploring both the experimental and analytical aspects of the subject, this practical reference guide is essential for researchers and students involved in material properties, whether in physics, materials science, metallurgy, or physical chemistry.

英语 [en] · PDF · 7.1MB · 2001 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

duxiu/initial_release/METHODS IN CELL BIOLOGY CORRELATIVE LIGHT AND ELECTRON MICROSCOPY II VOLUME 124_40800821.zip

METHODS IN CELL BIOLOGY CORRELATIVE LIGHT AND ELECTRON MICROSCOPY II VOLUME 124 THOMAS MULLER-REICHERT AND PAUL VERKADE, Thomas Müller-Reichert, Paul Verkade, Thomas Mueller-Reichert, Paul Verkade Elsevier Science & Technology Books, 2014, 2014

This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material Second of two volumes dedicated to Correlative Light and Electron microscopy (CLEM)

英语 [en] · PDF · 112.0MB · 2014 · 📗 未知类型的图书 · 🚀/duxiu/zlibzh · Save

lgli/A:\_for_add\1\SD\9780123957313 (24).pdf

The Use of Radioautography in Investigating Protein Synthesis C. Leblond (Eds.) Academic Press, Incorporated, Symposia of the International Society for Cell Biology, v.4, 1965

The Use of Radioautography in Investigating Protein Synthesis represents the 1965 Symposium on The Use of Radioautography in Investigating Protein Synthesis, supported by the International Society for Cell Biology. This book is composed of four parts encompassing 18 chapters. The opening part deals first with the basic aspects and mode of operation of the radioautography technique. This part also examines the advantages and potentials of this technique compared to other cytological techniques. The next parts are devoted to the experimental methods of protein synthesis in nucleus and in cells of various types, as revealed by the electron microscope radioautography. The concluding part discusses the secretion and synthesis of protein enzymes by the acinar cells in rat pancreas and fibroblasts in wound healing. This part also considers the application of radioautography to determining the sites of synthesis of proteins. This book is of great value to cell biologists, biochemists, cytologists, histologists, and pathologists.

英语 [en] · PDF · 20.6MB · 1965 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

lgli/A:\_for_add\1\SD\9780123188809 (14).pdf

Electron microscopy of plant cells. Ed. by J. L. Hall, C. Hawes C. Hawes (Eds.) Academic Press, Incorporated, Elsevier Ltd., London, 1991

Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

英语 [en] · PDF · 12.3MB · 1991 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

lgli/BOOKSMEDICOS.ORG - Ultrastructure Atlas of Human Tissues (2014, ).pdf

Ultrastructure Atlas of Human Tissues BOOKSMEDICOS.ORG Wiley-Blackwell, John Wiley & Sons, Inc., Hoboken, New Jersey, 2014

Ultrastructure Atlas of Human Tissues presents a variety of scanning and transmission electron microscope images of the major systems of the human body. Photography with the electron microscope records views of the intricate substructures and microdesigns of objects and tissues, and reveals details within them inaccessible to the naked eye or light microscope. Many of these views have significance in understanding normal structure and function, as well as disease processes. This book offers a unique and comprehensive look at the structure and function of tissues at the subcellular and molecular level, an important perspective in understanding and combating diseases. • Presents the major systems of the human body through scanning and transmission electron microscope images • Has images prepared almost exclusively from human tissues • Includes electron micrographs of common pathologies such as fibrotic and emphysemic lung, kidney stones, sickle cell anemia, and skin parasites • Contains sets of 3D images in most chapters

英语 [en] · PDF · 112.2MB · 2014 · 📘 非小说类图书 · 🚀/lgli/zlib · Save

ia/butterfliesofpap0000pars.pdf

Some Biological Techniques in Electron Microscopy Donald F Parsons; William G Banfield New York: Academic Press, Elsevier Ltd., New York, 1970

Some Biological Techniques in Electron Microscopy discusses the problems that hinder or prevent progress in biological electron microscopy. This book explores the great potential of electron microscope for solving several pressing medical and biological research problems. Organized into five chapters, this book starts with an overview of the primary goal of biological electron microscopy, which is the visualization of atoms in biological molecules and structures. This text proceeds with a discussion of the freezing methods of electron microscopy in which some of the artifacts producing steps of environmental techniques are eliminated. Other chapters consider the objective of electron microscopy to resolve successively small objects, in the limiting instance, single atoms in amorphous structures. The final chapter deals with processing tissues for electron microscopy wherein temperature, agitation, pressure, and humidity can be controlled throughout the operation. Biologists, biophysicists, physicists, electron microscopists, and medical researchers will find this book extremely useful.

英语 [en] · PDF · 11.2MB · 1970 · 📗 未知类型的图书 · 🚀/ia · Save

lgli/G:\!genesis\1\trans tech\Materials Science Forum, Volume 644 Advanced Electron Microscop.pdf

Advanced electron microscopy and nanomaterials : selected, peer reviewed papers from the First Joint Advanced Electron Microscopy School for Nanomaterials and the Workshop on Nanomaterials (AEM-NANOMAT '09), Saltillo (Coahuila) México, September 29th-October 2nd, 2009 Arturo Ponce; Darío Bueno Trans Tech Publications, Limited, Materials science forum, v. 644, 2010

Selected, peer reviewed papers from the First Joint Advanced Electron Microscopy School for Nanomaterials and the Workshop on Nanomaterials (AEM-NANOMAT'09), Saltillo (Coahuila) México, September 29th-October 2nd, 2009

英语 [en] · PDF · 37.9MB · 2010 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

nexusstc/Advances in Imaging and Electron Physics/2c7f25fd0ec99f7b822b109e221fbf2d.pdf

Advances in imaging and electron physics. Volume 159, The scanning transmission electron microscope Peter W. Hawkes (Eds.) Academic Press [Imprint], Elsevier Science & Technology Books, Advances in Imaging and Electron Physics, Advances in Imaging and Electron Physics 159, 1, 2009

Advances in Imaging and Electron Physics merges two long-running serials--Advances in Electronics and Electron Physics and Advances in Optical and Electron Microscopy. This series features extended articles on the physics of electron devices (especially semiconductor devices), particle optics at high and low energies, microlithography, image science and digital image processing, electromagnetic wave propagation, electron microscopy, and the computing methods used in all these domains. This particular volume presents several timely articles on the scanning transmission electron microscope. <li>Updated with contributions from leading international scholars and industry experts </li><li>Discusses hot topic areas and presents current and future research trends </li><li>Provides an invaluable reference and guide for physicists, engineers and mathematicians</li>

英语 [en] · PDF · 13.9MB · 2009 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

nexusstc/Practical Electron Microscopy of Lattice Defects/4315f95593f5b675621561767bf32eb3.pdf

Practical Electron Microscopy of Lattice Defects Hiroyasu Saka World Scientific Publishing Co Pte Ltd, World Scientific Publishing Company, Singapore, 2021

'Although the study of such defects is regularly examined at length in more general books on electron microscopy, this text in which they are centre-stage will surely be appreciated.' [Read Full Review]UltramicroscopyThis unique reference text provides those who are studying crystal lattice defects using a transmission electron microscope (TEM) with a basic knowledge of transmission electron microscopy. As it has been written for beginners, the principles of both transmission electron microscopy and crystallography have been clearly and simply explained, with the use of many figures and photographs to aid understanding. Mathematics is avoided where possible, and problems and exercises are amply provided.

英语 [en] · PDF · 35.5MB · 2021 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

lgli/1801.pdf

Funktionelle Ultrastruktur: Atlas der Biologie und Pathologie von Geweben (German Edition) Margit Pavelka, Jürgen Roth Springer-Verlag GmbH, Springer Nature, Wien, 2005

Mit dem Blick auf die Feinstrukturen werden die Innenräume der Zellen und ihre Architekturen in der höheren Organisation der Gewebe lebendig. Die Autoren geben mit diesem Atlas der funktionellen Ultrastruktur Einblick in die komplexe zelluläre Innenwelt mit ihren vielgestaltigen Funktionsräumen, sowie in dynamische Funktionsabläufe, die für die Erfüllung der zellulären Aufgaben im Gewebe notwendig sind. Das besondere Anliegen besteht darin, dem Leser bewusst zu machen, dass es sich hier um diejenigen Strukturen handelt, in denen sich grundlegende zelluläre Lebensvorgänge und krankmachende Mechanismen abspielen. An Hand elektronenmikroskopischer Bilder, ergänzt durch Immunelektronenmikroskopie und ausführlicher Beschreibung werden in den Zellen die Beziehungen zwischen Strukturen und Funktionen aufgerollt, Details herausgearbeitet und die Organisation in den Zellverbänden der Gewebe präsentiert. Das Buch wendet sich an Medizin- und Naturwissenschaftsstudenten, Ärzte, Biologen, Zell- und Molekularbiologen.

德语 [de] · PDF · 49.0MB · 2005 · 📘 非小说类图书 · 🚀/lgli/lgrs · Save

scihub/10.1007/978-3-642-72815-0.pdf

Cryotechniques in Biological Electron Microscopy || Luis Bachmann, Erwin Mayer (auth.), Prof. Dr. Rudolf Alexander Steinbrecht, Dr. Karl Zierold (eds.) Springer Berlin / Heidelberg, 10.1007/97, 1987

To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

英语 [en] · PDF · 55.4MB · 1987 · 📘 非小说类图书 · 🚀/lgli/scihub/zlib · Save

scihub/10.1007/978-94-009-5540-0.pdf

Electron Beam Analysis of Materials || M. H. Loretto (auth.) Springer London, Limited, 10.1007/97, 1984

The examination of materials using electron beam techniques has developed continuously for over twenty years and there are now many different methods of extracting detailed structural and chemical information using electron beams. These techniques which include electron probe microanalysis, trans mission electron microscopy, Auger spectroscopy and scanning electron microscopy have, until recently, developed more or less independently of each other. Thus dedicated instruments designed to optimize the performance for a specific application have been available and correspondingly most of the available textbooks tend to have covered the theory and practice of an individual technique. There appears to be no doubt that dedicated instru ments taken together with the specialized textbooks will continue to be the appropriate approach for some problems. Nevertheless the underlying electron-specimen interactions are common to many techniques and in view of the fact that a range of hybrid instruments is now available it seems appropriate to provide a broad-based text for users of these electron beam facilities. The aim of the present book is therefore to provide, in a reasonably concise form, the material which will allow the practitioner of one or more of the individual techniques to appreciate and to make use of the type of information which can be obtained using other electron beam techniques.

英语 [en] · PDF · 18.9MB · 1984 · 📘 非小说类图书 · 🚀/lgli/scihub/zlib · Save

ia/fixationforelect0000haya.pdf

Fixation for Electron Microscopy M.A. (Eric) Hayat Academic Press, Incorporated, Elsevier Ltd., Oxford, 1981

Fixation for Electron Microscopy presents how to better understand the effects of fixatives on the molecular structure of the cell. This book attempts to consider each aspect of fixation, including chemical interactions between fixatives and individual cellular substances. The chemistry of fixative interactions that are discussed in the book is based primarily on the reactions of a fixative with isolated proteins, lipids, nucleic acids, and carbohydrates. The book shows that the correct interpretation of information retrieved from electron micrographs depends on the knowledge of the basic principles underlying the fixation procedure. Also, the book presents the fixation of both eukaryotic and prokaryotic specimens. The special fixation conditions for plant specimens are discussed in detail and have been allotted a whole chapter. Also emphasized in this book is the connection between morphology and biochemical aspects of preparatory treatments and the chemical basis of the formation of artifacts. This topic is useful in understanding the modifications of cell structures introduced during their processing. A guide for recognizing and minimizing major artifacts and fixation faults that are usually encountered is also presented in the book. This valuable resource will prove useful to both students and professionals in the field of biology and clinical medicine. Specimen preservation researchers can also benefit from this book.

英语 [en] · PDF · 34.1MB · 1981 · 📗 未知类型的图书 · 🚀/ia · Save

ia/beginningsofelec0000unse.pdf

The Beginnings of Electron Microscopy edited by Peter W. Hawkes Academic Press, Incorporated, Elsevier Ltd., Orlando, 1985

The Beginnings of Electron Microscopy presents the technical development of electron microscope. This book examines the mechanical as well as the technical problems arising from the physical properties of the electron. Organized into 19 chapters, this book begins with an overview of the history of scanning electron microscopy and electron beam microanalysis. This text then explains the applications and capabilities of electron microscopes during the war. Other chapters consider the classical techniques of light microscopy. This book presents as well the schematic outline of the preparation techniques for investigation of nerve cells by electron microscopy. The final chapter deals with the historical account of the beginnings of electron microscopy in Russia. This book is a valuable resource for scientists, technologists, physicists, electrical engineers, designers, and technicians. Graduate students as well as researcher workers who are interested in the history of electron microscopy will also find this book extremely useful.

英语 [en] · PDF · 39.0MB · 1985 · 📗 未知类型的图书 · 🚀/ia · Save

lgli/A:\compressed\10.1007%2F978-1-4615-6887-2.pdf

Electron Energy-Loss Spectroscopy in the Electron Microscope Ray F. Egerton (auth.) Springer US, 1, 1995

Electron energy-loss spectroscopy (EELS or ELS) has been used to investi gate the physical properties of solids for over 40 years in a handful of laboratories distributed around the world. More recently, electron micro scopists have become interested in EELS as a method of chemical analysis with the potential for achieving very high sensitivity and spatial resolution, and there is a growing awareness of the fact that the loss spectrum can provide structural information from a thin specimen. In comparison with energy-dispersive x-ray spectroscopy, for example, EELS is a fairly demand ing technique, requiring for its full exploitation a knowledge of atomic and solid-state physics, electron optics, and electronics. In writing this book, I have tried to gather together relevant information from these various fields. Chapter 1 begins at an elementary level; readers with some experience in EELS will be familiar with the content of the first two sections. Chapter 2 deals with instrumentation and experimental technique, and should con tain material of interest to researchers who want to get the best performance out of commercial equipment as well as those who contemplate building their own spectrometer or electron-detection system. Chapter 3 outlines the theory used to interpret spectral features, while Chapter 4 gives procedures for numerical processing of the energy-loss spectrum. Chapter 5 contains examples of practical applications of EELS and a discussion of radiation damage, spatial resolution, and detection limits.

英语 [en] · PDF · 11.6MB · 1995 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save

lgli/A:\sciencedirect_books\10765670-173 (16).pdf

Advances In Imaging And Electron Physics, Volume 173: Part B Jay Theodore Cremer (Eds.) Academic Press, Incorporated, Advances in Imaging and Electron Physics 173, 1, 2012

This special volume of Advances in Imaging and Electron Physics details the current theory, experiments, and applications of neutron and x-ray optics and microscopy for an international readership across varying backgrounds and disciplines. Edited by Dr. Ted Cremer, these volumes attempt to provide rapid assimilation of the presented topics that include neutron and x-ray scatter, refraction, diffraction, and reflection and their potential application. Key features: * Contributions from leading authorities * Informs and updates on all the latest developments in the field

英语 [en] · PDF · 4.1MB · 2012 · 📘 非小说类图书 · 🚀/lgli/lgrs/zlib · Save

nexusstc/Electron Microscopy Methods and Protocols (Methods in Molecular Biology)/3715d3540958839b6104570f61dd2353.pdf

Electron Microscopy Methods and Protocols Volume 117 || High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling M.A. Nasser Hajibagheri (editor) Humana Press; Brand: Humana Press, Springer Nature, Totowa, N.J., 1999

Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual. Disc. wide range of applications for the examination of cells, tissues, macromolecules, structures, interactions.

英语 [en] · PDF · 4.8MB · 1999 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/scihub · Save

upload/newsarch_ebooks/2020/01/16/1107124123.pdf

Wavefront Shaping for Biomedical Imaging (Advances in Microscopy and Microanalysis) Kubby, Joel (editor);Gigan, Sylvain (editor);Cui, Meng (editor) Cambridge University Press (Virtual Publishing), Advances in Microscopy and Microanalysis, 2019 may 31

Learn about the theory, techniques and applications of wavefront shaping in biomedical imaging using this unique text. With authoritative contributions from researchers who are defining the field, cutting-edge theory is combined with real-world practical examples, experimental data and the latest research trends to provide the first book-level treatment of the subject. It is suitable for both background reading and use in a course, with coverage of essential topics such as adaptive optical microscopy, deep tissue microscopy, time reversal and optical phase conjugation, and tomography. The latest images from the forefront of biomedical imaging are included, and full-colour versions are available in the eBook version. Researchers, practitioners and graduate students in optics, biophotonics, biomedical engineering, and biology who use biomedical imaging tools and are looking to advance their knowledge of the subject will find this an indispensable resource.

英语 [en] · PDF · 21.3MB · 2019 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/upload/zlib · Save

ia/nanocharacterisa0000unse.pdf

Nanocharacterisation (RSC Nanoscience and Nanotechnology Series) (RSC Nanoscience and Nanotechnology Series) John Hutchison; Angus Kirkland Royal Society of Chemistry, Lightning Source Inc. (Tier 3), Cambridge, 2007

Chemical characterisation techniques have been essential tools in underpinning the explosion in nanotechnology in recent years and nanocharacterisation is a rapidly developing field. Contributions in this book from leading teams across the globe provide an overview of the different microscopic techniques now in regular use for the characterisation of nanostructures. Essentially a handbook to all working in the field this indispensable resource provides a survey of microscopy based techniques with experimental procedures and extensive examples of state of the art characterisation methods including: Transmission Electron Microscopy, Electron Tomography, Tunneling Microscopy, Electron Holography, Electron Energy Loss Spectroscopy. This timely publication will appeal to academics, professionals and anyone working fields related to the research and development of nanocharacterisation and nanotechnology.

英语 [en] · PDF · 19.7MB · 2007 · 📗 未知类型的图书 · 🚀/ia · Save

nexusstc/Electro Microscopy Methods and Protocols (Methods in Molecular Biology Vol 117)/a67473986cb8860f40ef0fe5dc36890d.epub

Electron Microscopy Methods And Protocols. Methods In Molecular Biology, Volume 117 edited by M.A. Nasser Hajibagheri Springer, 2014

Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques - each optimized for reproducibility and robust results - today's gold-standard laboratory manual.

英语 [en] · EPUB · 4.1MB · 2014 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save