The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods

lgrsnf/A:\_for_add\1\SD\series\0091679X-111 (26).pdf

nexusstc/Correlative Light and Electron MIcroscopy/a0e3c142bd48a2c5b9fd169ed3694187.pdf

zlib/Biology and other natural sciences/Molecular/Thomas Müller-Reichert and Paul Verkade (Eds.)/Correlative Light and Electron MIcroscopy_2212275.pdf

ed. by Thomas Müller-Reichert, Paul Verkade

Thomas Mueller-Reichert, Paul Verkade

Mueller-Reichert, Thomas

Müller-Reichert, Thomas

Elsevier Science & Technology Books

Academic Press, Incorporated

Morgan Kaufmann Publishers

Elsevier/Academic Press

Brooks/Cole

Methods in cell biology -- vol. 111, Kidlington, Oxford, United Kingdom, 2012

Methods in cell biology, v. 111, 1st ed, Amsterdam, Netherlands, 2012

Elsevier Ltd., Amsterdam, Netherlands, 2012

United States, United States of America

lg1043194

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Content:
Series Editors
Page ii
Front Matter
Page iii
Copyright
Page iv
Contributors
Pages xi-xv
Preface
Pages xvii-xix
Thomas Müller-Reichert, Paul Verkade
Chapter 1 - Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
Pages 1-20
K. Kobayashi, D. Cheng, M. Huynh, K.R. Ratinac, P. Thordarson, F. Braet
Chapter 2 - Visualizing Live Dynamics and Ultrastructure of Intracellular Organelles with Preembedding Correlative Light-Electron Microscopy
Pages 21-35
Roman S. Polishchuk, Elena V. Polishchuk, Alberto Luini
Chapter 3 - Correlative Fluorescence and Transmission Electron Microscopy in Tissues
Pages 37-57
Toshihiro Takizawa, John M. Robinson
Chapter 4 - Correlative Light and Electron Microscopy in Parasite Research
Pages 59-73
Céline Loussert, Claire-Lise Forestier, Bruno M. Humbel
Chapter 5 - Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
Pages 75-93
Gunar Fabig, Susanne Kretschmar, Susanne Weiche, Dominic Eberle, Marius Ader, Thomas Kurth
Chapter 6 - 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
Pages 95-115
Katia Cortese, Giuseppe Vicidomini, Maria Cristina Gagliani, Patrizia Boccacci, Alberto Diaspro, Carlo Tacchetti
Chapter 7 - Correlative Light and Electron Microscopy of GFP
Pages 117-138
Markus Grabenbauer
Chapter 8 - Picking Faces out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
Pages 139-155
Mark H. Ellisman, Thomas J. Deerinck, Xiaokun Shu, Gina E. Sosinsky
Chapter 9 - Correlated Light Microscopy and Electron Microscopy
Pages 157-173
Klaas A. Sjollema, Ulrike Schnell, Jeroen Kuipers, Ruby Kalicharan, Ben N.G. Giepmans
Chapter 10 - Capturing Endocytic Segregation Events with HPF-CLEM
Pages 175-201
Edward Brown, Jan Van Weering, Thom Sharp, Judith Mantell, Paul Verkade
Chapter 11 - Targeted Ultramicrotomy: A Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
Pages 203-222
Irina Kolotuev, Daniel J. Bumbarger, Michel Labouesse, Yannick Schwab
Chapter 12 - Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo
Pages 223-234
Ina Woog, Silke White, Mandy Büchner, Martin Srayko, Thomas Müller-Reichert
Chapter 13 - Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
Pages 235-257
Wanda Kukulski, Martin Schorb, Sonja Welsch, Andrea Picco, Marko Kaksonen, John A.G. Briggs
Chapter 14 - Integrative Approaches for Cellular Cryo-electron Tomography: Correlative Imaging and Focused Ion Beam Micromachining
Pages 259-281
Alexander Rigort, Elizabeth Villa, Felix J.B. Bäuerlein, Benjamin D. Engel, Jürgen M. Plitzko
Chapter 15 - Visualizing Proteins in Electron Micrographs at Nanometer Resolution
Pages 283-306
Shigeki Watanabe, Erik M. Jorgensen
Chapter 16 - Atmospheric Scanning Electron Microscope for Correlative Microscopy
Pages 307-324
Ian E.G. Morrison, Clare L. Dennison, Hidetoshi Nishiyama, Mitsuo Suga, Chikara Sato, Andrew Yarwood, Peter J. O’Toole
Chapter 17 - Bridging Microscopes: 3D Correlative Light and Scanning Electron Microscopy of Complex Biological Structures
Pages 325-356
Miriam S. Lucas, Maja Günthert, Philippe Gasser, Falk Lucas, Roger Wepf
Chapter 18 - Correlative Light and Volume Electron Microscopy: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms
Pages 357-382
Andrew J. Bushby, Giovanni Mariggi, Hannah E.J. Armer, Lucy M. Collinson
Index
Pages 383-393
Volumes in Series
Pages 395-404
Colour Plate
Pages 1-40

<p>The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; <b>CLEM</b>) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of <i>Methods in Cell Biology</i> covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in <i>C. elegans</i> embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.</p><br><br><li>Covers many areas of CLEM by the best international scientists in the field</li><li>Includes a brief history and overview on CLEM methods</li>

"The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM"--Publisher

2013-11-12