The revised and expanded second edition of this book presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

lgrsnf/MMB/MMB vol.369 Kuo J. (ed.) Electron Microscopy Methods and Protocols (Humana Press, 2007)(ISBN 1588295737)(625s).pdf

nexusstc/Electron Microscopy/12e184781229608f3abd23ecbf8d3431.pdf

scihub/10.1007/978-1-59745-294-6.pdf

zlib/Biology and other natural sciences/John J. Bozzola (auth.), John Kuo (eds.)/Electron Microscopy: Methods and Protocols_657671.pdf

Electron Microscopy: Methods And Protocols (methods In Molecular Biology)

John Kuo; NetLibrary, Inc

Kuo, John

Humana P.,u.s.

Methods in molecular biology (Clifton, N.J.), v. 369, 2nd ed. /, Totowa, N.J, ©2007

Methods in molecular biology, 2nd ed, Totowa, cop. 2007

United States, United States of America

Springer Nature, Totowa, N.J., 2007

2nd ed. edition, February 27, 2007

2nd, PS, 2007

2, 2006

kolxoz-dop

sm21556402

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Rev. ed. of: Electron microscopy methods and protocols / edited by M.A. Nasser Hajibagheri. c1999
Includes bibliographical references and index

Comprises Two Major Areas Of Electron Microscopy-transmission Electron Microscopy (tem) And Scanning Electron Microscopy (sem). The Tem Area Covers Several Key Techniques, Including: Conventional Specimen Preparation Methods For Cultured Cells And Biomedical And Plant Tissues; Cryospecimen Preparation By High-pressure Freezing And Cryoultramicrotomy Negative Staining And Immunogold Labeling Techniques; And Tem Crystallography And Cryo-tem Tomography. The Sem Area Similarly Attends To Conventional-, Variable Pressure-, Environmental-, And Cryoscanning Microscopy Techniques, As Well As The Application Of X-ray Microanalysis. Protocols For The Application Of X-ray Microanalysis To Sem And Mass Spectrometry Conclude The Volume. Conventional Specimen Preparation Techniques For Transmission Electron Microscopy Of Cultured Cells / John J. Bozzola -- Processing Biological Tissues For Ultrastructural Study / José A. Mascorro And John J. Bozzola -- Processing Plant Tissues For Ultrastructural Study / John Kuo And Ivan Kuo -- Microwave-assisted Processing And Embedding For Transmission Electron Microscopy / Paul Webster -- Ultramicrotomy For Biological Electron Microscopy / Herbert K. Hagler -- Poststaining Grids For Transmission Electron Microscopy : Conventional And Alternative Protocols / E. Ann Ellis -- Negative Staining Of Thinly Spread Biological Samples / J. Robin Harris. Correlative Light And Electron Microscopy Using Immunolabeled Resin Sections / Heinz Schwarz And Bruno M. Humbel -- Cryosectioning Fixed And Cryoprotected Biological Material For Immunocytochemistry / Paul Webster And Alexandre Webster -- F-tem Images : Integrating Fluorescence Microscopy With Transmission Electron Microscopy / Paul A. Sims And Jeff D. Hardin -- Quantitative Immunoelectron Microscopy : Alternative Ways Of Assessing Subcellular Patterns Of Gold Labelling / Terry M. Mayhew -- Electron Crystallography Of Membrane Proteins / Hui-ting Chou, James Evans, And Henning Stahlberg. Cryo-electron Microscopy Of Icosahedral Virus Particle / Wen Jiang And Wah Chiu -- 3d Reconstructions Of Chromosomes With Electron Tomography / Peter Engelhardt -- Whole-mount Immuno-electron Tomography Of Chromosomes And Cells / Peter Engelhardt ... [et Al.] -- 3d Cryo-transmission Electron Microscopy Of Cells And Organelles / Michael Marko And Chyong-ere Hsieh -- Electron Energy-loss Spectroscopy (eels) As A Tool For Elemental Analysis In Biological Specimens / Nadine Kapp ... [et Al.] -- Conventional Specimen Preparation Techniques For Scanning Electron Microscopy Of Biological Specimens / John J. Bozzola. Recent Advances In High-pressure Freezing : Equipment- And Specimen-loading Methods / Kent L. Mcdonald ... [et Al.] -- Cryo-ultramicrotomy : Cryo-electron Microscopy Of Vitreous Sections / Dimitri Vanhecke, Luca Studer, And Daniel Studer -- Cell-free Extract Systems And The Cytoskeleton : Preparation Of Biochemical Experiments For Transmission Electron Microscopy / Margaret Coughlin, William M. Brieher, And Ryoma Ohi -- Electron Microscopy/in Situ Hybridization Tracking Of Dna And Rna Sequences At High Resolution / Dusan Cmarko And Karel Koberna. Using Sims And Mims In Biological Materials : Application To Higher Plants / Nicole Grignon. Variable Pressure And Environmental Scanning Electron Microscopy : Imaging Of Biological Samples / Brendan J. Griffin -- Cryoplaning Technique For Visualizing The Distribution Of Water In Woody Tissue By Cryo-scanning Electron Microscopy / Yasuhiro Utsumi And Yuzou Sano -- X-ray Microanalysis In The Scanning Electron Microscope / Godfrield M. Roomans And Anca Dragomir -- Botanical Sem X-ray Microanalysis In Cryo-scanning Electron Microscopy / Beat Frey -- Static Secondary Ion Mass Spectrometry For Biological And Biomedical Research / Nicholas P. Lockyer. Edited By John Kuo. Rev. Ed. Of: Electron Microscopy Methods And Protocols / Edited By M.a. Nasser Hajibagheri. C1999. Includes Bibliographical References And Index.

Conventional specimen preparation techniques for transmission electron microscopy of cultured cells / John J. Bozzola
Processing biological tissues for ultrastructural study / José A. Mascorro and John J. Bozzola
Processing plant tissues for ultrastructural study / John Kuo
Microwave-assisted processing and embedding for transmission electron microscopy / Paul Webster
Ultramicrotomy for biological electron microscopy / Herbert K. Hagler
Poststaining grids for transmission electron microscopy : conventional and alternative protocols / E. Ann Ellis
Negative staining of thinly spread biological samples / J. Robin Harris
Recent advances in high-pressure freezing : equipment- and specimen-loading methods / Kent L. McDonald ... [et al.]
Cryoultramicrotomy : cryoelectron microscopy of vitreous sections / Dimitri Vanhecke, Luca Studer, and Daniel Studer
Cell-free extract systems and the cytoskeleton : preparation of biochemical experiments for transmission electron microscopy / Margaret Coughlin, William M. Brieher, and Ryoma Ohi
Electron microscopy in situ hybridization : tracking of DNA and RNA sequences at high resolution / Dušan Cmarko and Karel Koberna
Correlative light and electron microscopy using immunolabeled resin sections / Heinz Schwarz and Bruno M. Humbel
Cryosectioning fixed and cryoprotected biological material for immunocytochemistry / Paul Webster and Alexandre Webster
Fluorescence-integrated transmission electron microscopy images : integrating fluorescence microscopy with transmission electron microscopy / Paul A. Sims and Jeff D. Hardin
Quantitative immunoelectron microscopy : alternative ways of assessing subcellular patterns of gold labelling / Terry M. Mayhew
Electron crystallography of membrane proteins / Hui-Ting Chou, James E. Evans, and Henning Stahlberg
Cryoelectron microscopy of icosahedral virus particles / Wen Jiang and Wah Chiu
Three-dimensional reconstruction of chromosomes using electron tomography / Peter Engelhardt
Whole-mount immunoelectron tomography of chromosomes and cells / Peter Engelhardt ... [et al.]
Three-dimensional cryotransmission electron microscopy of cells and organelles / Michael Marko and Chyong-Ere Hsieh
Electron energy-loss spectroscopy as a tool for elemental analysis in biological specimens / Nadine Kapp ... [et al.]
Conventional specimen preparation techniques for scanning electron microscopy of biological specimens / John J. Bozzola
Variable pressure and environmental scanning electron microscopy : imaging of biological samples / Brendan J. Griffin
Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy / Yasuhiro Utsumi and Yuzou Sano
X-ray microanalysis in the scanning electron microscope / Godfrield M. Roomans and Anca Dragomir
Botanical X-ray microanalysis in cryoscanning electron microscopy / Beat Frey
Static secondary ion mass spectrometry for biological and biomedical research / Nicholas P. Lockyer
Using SIMS and MIMS in biological materials : application to higher plants / Nicole Grignon

In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Each protocol is presented by experts with years of first-hand experience and in-depth knowledge of the techniques they describe. The Notes section accompanying each protocol will prove invaluable for adapting and troubleshooting protocols. Electron Microscopy: Methods and Protocols, Second Edition, comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

Front Matter....Pages i-xv
Back Matter....Pages 1-18
....Pages 19-34

2010-02-18