Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. Updates the numerous technological innovations that have improved the capabilities of electron microscopy Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging Chapters include a balance of'how to','so what'and'where next', providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

McIntosh, J. Richard

Academic Press, Incorporated

Morgan Kaufmann Publishers

Brooks/Cole

Methods in cell biology -- v. 79, Amsterdam, Boston, Netherlands, 2007

Methods in cell biology, Amsterdam, cop. 2007

United States, United States of America

1 edition, February 22, 2007

Volume 79, 2007

obscured text on cover

Includes bibliographical references and index.

The Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells -- J. Dubochet
Cryopreparation Methods for Electron Microscopy of Selected Model Systems -- K. McDonald
Cryopreparation Methodology for Plant Cell Biology -- M. W. Hess
Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis -- T. Muller-Reichert
Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography -- E. T. O'Toole
Whole-Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography -- J. L. Hoog
Electron Microscopy of Archaea -- C. E. Robertson
Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography -- B. J. Marsh
Visualization of Membrane-Cytoskeletal Interactions During Plant Cytokinesis -- M. S. Otegui
Electron Tomographic Methods for Studying the Chemical Synapse -- C. P. Arthur
Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle -- B. F. McEwen
Electron Microscopic Analysis of the Leading Edge in Migrating Cells -- T. Svitkina
Imaging Actomyosin In Situ -- K. A. Taylor
Electron Tomography of Bacterial Chemotaxis Receptor Assemblies -- P. Zhang
How to "Read" a Vitreous Section -- J. Dubochet
Single-Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction -- I. I. Serysheva
Electron Microscopy of Microtubule-Based Cytoskeletal Machinery -- A. Hoenger
Reconstructing the Endocytotic Machinery -- Y. Cheng
3D Immunolocalization with Plastic Sections -- M. K. Morphew
Electron Microscopy Analysis of Viral Morphogenesis -- A. Pelchen-Matthews
Electron Tomography of Immunolabeled Cryosections -- M. S. Ladinsky
Visualizing Macromolecules with Fluoronanogold: From Photon Microscopy to Electron Tomography -- T. Cheutin
Markers for Correlated Light and Electron Microscopy -- G. E. Sosinsky
Localizing Specific Elements Bound to Macromolecules by EFTEM -- R. D. Leapman
Localization of Protein Complexes by Pattern Recognition -- C. Best
The Application of Energy-Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples -- J. C. Bouwer
Optimization of Image Collection for Cellular Electron Microscopy -- P. Mooney
Future Directions for Camera Systems in Electron Microscopy -- N. Xuong
Structure Determination In Situ by Averaging of Tomograms -- F. Forster
Methods for Image Segmentation in Cellular Tomography -- K. Sandberg
Database Resources for Cellular Electron Microscopy -- M. E. Martone.

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.<br><br>* Updates the numerous technological innovations that have improved the capabilities of electron microscopy<br>* Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging<br>* Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

"Recent improvements in electron microscopy of cells are bringing cell biologists back to "EM", particularly as they realize the limitations of the light microscopic methods that have been dominating the study of cell structure. This trend is a direct result of the resolving power of the electron microscope, of improved methods for specimen preparation, and of new technologies for three-dimensional imaging and image analysis. EM is beginning to fulfill its promise of linking light microscopy of live cells with atomic resolution studies of isolated macromolecules, with the goal of improving our understanding of living systems at atomic resolution. These new methods will help biologists from many disciplines to apply modern EM to their own work, allowing them to participate in the groundbreaking discoveries that are now being made in this field." "Cellular Electron Microscopy is divided into five sections, each beginning with an introduction by the editor. The sections cover many issues: specimen preparation, 3D imaging, use of frozen-hydrated samples, labeling macromolecules, and technologies for data aquisition and analysis. This book is explicitly designed for new and potential users, not experts in EM, so the chapters focus on practical information, including discussions of why each topic is important and its likely future developments. The work will prove an invaluable resource for anyone in cell biology, as well as those in biophysics, microbiology, neuroscience, microscopy, biochemistry, and genetics. Book jacket."--BOOK JACKET

Advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book helps biologists from many disciplines understand modern EM and the value it might bring to their own work.

xxvii, 850 p., [28] p. of plates :
Includes bibliographical references and index

2023-06-28