This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful __Methods in Molecular Biology__ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, __Erythropoiesis: Methods and Protocols__ provides a definitive source for molecular and cellular biology protocols to study erythropoiesis and to move this vital field forward.

lgli/K:\!genesis\0day\springer\10.1007%2F978-1-4939-7428-3.pdf

lgrsnf/K:\!genesis\0day\springer\10.1007%2F978-1-4939-7428-3.pdf

nexusstc/Erythropoiesis: Methods and Protocols/f5f0466f92f20e9914f7c7ffb5a0c14e.pdf

scihub/10.1007/978-1-4939-7428-3.pdf

zlib/Biology and other natural sciences/Joyce A. Lloyd (eds.)/Erythropoiesis: Methods and Protocols_3394086.pdf

Adobe InDesign CC 2017 (Windows)

Methods in molecular biology, 1698, 1st ed. 2018, New York, NY, 2018

Methods in molecular biology (Clifton, N.J.), New York, NY, 2018

Methods in molecular biology (Clifton, N.J.), 2017

Methods in Molecular Biology, 1698, New York, 2017

United States, United States of America

1st ed. 2018, 2017

sm66825540

producers:
Adobe PDF Library 15.0

{"container_title":"Methods in Molecular Biology","edition":"1","isbns":["1493974270","1493974289","9781493974276","9781493974283"],"issns":["1064-3745","1940-6029"],"publisher":"Springer New York","series":"Methods in Molecular Biology 1698"}

Front Matter ....Pages i-xi
An Introduction to Erythropoiesis Approaches (Joyce A. Lloyd)....Pages 1-10
Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis (Martin D. Kafina, Barry H. Paw)....Pages 11-36
Mouse Models of Erythropoiesis and Associated Diseases (Matthew P. Parker, Kenneth R. Peterson)....Pages 37-65
Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells (Skye C. McIver, Kyle J. Hewitt, Xin Gao, Charu Mehta, Jing Zhang, Emery H. Bresnick)....Pages 67-89
Stress Erythropoiesis Model Systems (Laura F. Bennett, Chang Liao, Robert F. Paulson)....Pages 91-102
Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models (Marie Trudel, Josepha-Clara Sedzro)....Pages 103-115
Functional Analysis of Erythroid Progenitors by Colony-Forming Assays (James Palis, Anne Koniski)....Pages 117-132
Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands (Jia Hao Yeo, Matthew P. Cosgriff, Stuart T. Fraser)....Pages 133-152
Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells (Xiuli An, Lixiang Chen)....Pages 153-174
Analysis of Erythropoiesis Using Imaging Flow Cytometry (Theodosia Kalfa, Kathleen E. McGrath)....Pages 175-192
Flow Cytometric Analysis of Erythroblast Enucleation (Xiuli An, Lixiang Chen)....Pages 193-203
High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure (Alyson S. Smith, Roberta B. Nowak, Velia M. Fowler)....Pages 205-228
Chromatin Immunoprecipitation (ChIP) with Erythroid Samples (Ivan Krivega, Ann Dean)....Pages 229-236
Chromosome Conformation Capture (3C and Higher) with Erythroid Samples (Ivan Krivega, Ann Dean)....Pages 237-243
Genome Editing of Erythroid Cell Culture Model Systems (Jinfen J. Yik, Merlin Crossley, Kate G. R. Quinlan)....Pages 245-257
In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood (Anna Kovilakath, Safa Mohamad, Farrah Hermes, Shou Zhen Wang, Gordon D. Ginder, Joyce A. Lloyd)....Pages 259-274
Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines (Divya S. Vinjamur, Daniel E. Bauer)....Pages 275-284
Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products (Neil W. A. McGowan, John D. M. Campbell, Joanne C. Mountford)....Pages 285-292
Back Matter ....Pages 293-298

This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successfulthods in Molecular Biology㥲ies, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, 岹ythropoiesis: Methods and Protocolsಯvides a definitive source for molecular and cellular biology protocols to study erythropoiesis and to move this vital field forward

Methods in Molecular Biology
Erscheinungsdatum: 27.10.2017

2017-11-21