This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area.

lgrsnf/A:\compressed\10.1007%2F978-1-4757-1257-5.pdf

nexusstc/Methods of Protein Separation/f3acd508e4679a42fcab28f1843a8f47.pdf

zlib/Biology and other natural sciences/Nicholas Catsimpoolas/Methods of Protein Separation_2137166.pdf

Biological separations, New York, New York, 1975

Place of publication not identified, 1976

Biological Separations, Boston, MA, 1975

United States, United States of America

Springer Nature, New York, NY, 2013

Oct 15, 1976

lg983240

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Source title: Methods of Protein Separation (Biological Separations)

This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "ConƯ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subseƯ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and thereƯ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g., chemically modified, deriving from a specific tissue, conjugated to different moieties, etc.) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area

Front Matter....Pages I-XIII
Sedimentation and Gel-Permeation Chromatography of Associating-Dissociating Macromolecules....Pages 1-25
Trans Electrophoresis....Pages 27-67
Immunodiffusion....Pages 69-92
Isoelectric Focusing in Polyacrylamide Gel....Pages 93-126
Purification of Chemically Modified Proteins....Pages 127-160
Chromatographic Peak Shape Analysis....Pages 161-192
Sedimentation Equilibrium of Proteins in Density Gradients....Pages 193-237
Hollow-Fiber Separation Devices and Processes....Pages 239-254
Affinity Chromatography, Principles and Applications....Pages 255-276
Back Matter....Pages 277-281

2013-08-01