Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.

lgrsnf/MMB/MMB vol.403 Molnar P., Hickman J.J. Patch-Clamp. Methods and Protocols (Humana Press, 2007)(ISBN 1588296989)(319s).pdf

nexusstc/Patch-Clamp Methods and Protocols/e0929bcd4ffb8a8931025fc74992b938.pdf

scihub/10.1007/978-1-59745-529-9.pdf

zlib/Medicine/László Fodor, József Nagy (auth.), Peter Molnar, James J. Hickman (eds.)/Patch-Clamp Methods and Protocols_657702.pdf

Peter Molnaŕ; James J Hickman; Bruno Allard

edited by Peter Molnar and James J. Hickman

Peter Molnár; James J Hickman

Humana ; [Quantum, distributor

Methods in molecular biology -- 403, Methods in molecular biology -- 403., Totowa, N.J, New Jersey, 2007

Methods in molecular biology (Clifton, N.J.), v. 403, Totowa, N.J., [Paisley, ©2007

Methods in molecular biology, 403, 1st ed. 2007, Totowa, NJ, 2007

Methods in molecular biology, 403, Totowa, NJ, 2010

United States, United States of America

1 edition, September 19, 2007

2007, 2007-09-19

kolxoz-dop

sm18449151

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Includes bibliographical references and index.

Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA receptors using the whole-cell patch-clamp method / László Fodor and József Nagy
Memantine as an example of a fast, voltage-dependent, open channel N-methyl-D-aspartate receptor blocker / Chris G. Parsons and Kate Gilling
Methods for evaluation of positive allosteric modulators of glutamate AMPA receptors / Edward R. Siuda, Jennifer C. Quirk, and Eric S.Nisenbaum
Automated voltage-clamp technique / Andrea Ghetti, António Guia, and Jia Xu
Flip-the-tip : automated patch clamping based on glass electrodes / Michael Fejtl ... [et al.]
The roboocyte : automated electrophysiology based on Xenopus oocytes / Christine Leisgen, Mike Kuester, and Christoph Methfessel
Infrared-guided laser stimulation as a tool for elucidating the synaptic site of expression of long-term synaptic plasticity / Gerhard Rammes ... [et al.]
Single-cell RT-PCR, a technique to decipher the electrical, anatomical, and genetic determinants of neuronal diversity / Maria Toledo-Rodriguez and Henry Markram
Mechanosensitive ion channels investigated simultaneously by scanning probe microscopy and patch clamp / Matthias G. Langer
Synaptic connectivity in engineered neuronal networks / Peter Molnar ... [et al.]
Modeling of action potential generation in NG108-15 cells / Peter Molnar and James J. Hickman
Whole-cell voltage clamp on skeletal muscle fibers with the silicone-clamp technique / Sandrine Pouvreau ... [et al.]
Determination of channel properties at the unitary level in adult mammalian isolated cardiomyocytes / Romain Guinamard
Electrophysiological properties of embryonic stem cells during differentiation into cardiomyocyte-like cell types / Antoni C.G. van Ginneken and Arnoud C. Fijnvandraat
Hybrid neuronal network studies under dynamic clamp / Alan D. Dorval II ... [et al.]
Cardiac channelopathies studied with the dynamic action potential-clamp technique / Géza Berecki ... [et al.]
Principles of single-channel kinetic analysis / Feng Qin
Use of Xenopus oocytes to measure ionic selectivity of pore-forming peptides and ion channels / Thierry Cens and Pierre Charnet
Estimation of quantal parameters with multiple-probability fluctuation analysis / Chiara Saviane and Robin Angus Silver.

Patch-Clamp Methods and Protocols surveys the typical patch-clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch-clamp technique effectively. Patch-Clamp Methods and Protocols is divided into three sections that cover the major areas of patch-clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step-by-step instructions on how to use whole-cell and single-channel patch-clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch-clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch-clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch-Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch-clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies. Features: Details how electrophysiology can be best utilized in drug discovery and development, Introduces applications of the dynamic damp method, Includes an extensive theoretical treatise concerning single channel kinetic analysis

Front Matter....Pages i-xi
Front Matter....Pages 1-1
Front Matter....Pages 3-13
Front Matter....Pages 15-36
Back Matter....Pages 37-57
....Pages 59-69

2010-02-18