A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing.
Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use.
Key Features
* Provides general guidelines and strategies for isolation of membrane proteins
* Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs
* Gives special emphasis to new native and denaturing electrophoresis techniques
* Explains modifications of techniques used for water-soluble proteins
Audience: Researchers, technicians, and students in academic and industrial laboratories working in protein and membrane biochemistry, biotechnology, cell biology, developmental biology, physiology, pharmacology, molecular biology, microbiology, immunology, neuroscience, and biophysics.

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zlib/Science (General)/Gebhard von Jagow, Arnold Revzin/A Practical Guide to Membrane Protein Purification, Volume 2_2295075.pdf

A Practical Guide to Membrane Protein Purification (Separation, Detection, and Characterization of Biological Macromolecules) (Separation, Detection, and Characterization of Biological Macromolecules)

Gebhard von Jagow; Hermann Schagger; Hermann Schägger

edited by Gebhard von Jagow, Hermann Schagger

Morgan Kaufmann Publishers

Elsevier Science

Brooks/Cole

Separation, detection, and characterization of biological macromolecules, San Diego, California, 1994

Separation, Detection, and Characterization of Biological Macromolecules, Burlington, 2013

United States, United States of America

Elsevier Ltd., San Diego, 1994

Spi edition, November 7, 1994

0

lg1126351

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Includes bibliographical references and index.

Intended especially for researchers who have some familarity with separation of water-soluble proteins, this guide emphasizes the aspects unique to membrane proteins. It also explains the principles of the methods, for researchers and students new to this area, to adapt these techniques to their particular needs.

2014-01-07