Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In __Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants__, expert researchers provide detailed, step-by-step protocols for the establishment of __in vitro__ cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of __in vitro__ germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the __Methods in Molecular Biology__TM series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, __Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants__ is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.

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zlib/Biology and other natural sciences/Biotechnology/Kee-Yoeup Paek, Hosakatte Niranjana Murthy, Eun-Joo Hahn (auth.), Praveen K. Saxena, S. Mohan Jain (eds.)/Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants_611178.pdf

edited by S. Mohan Jain and Praveen K. Saxena

Shri Mohan Jain; Praveen K. Saxena

Adobe Acrobat 7.0

Methods in molecular biology -- v. 547, Springer protocols, New York, New York State, 2009

Methods in molecular biology (Clifton, N.J.), 547, New York, NY, cop. 2009

Methods in molecular biology (Clifton, N.J.), v. 547, New York, NY, ©2009

United States, United States of America

torrents.ru tech collections 2009-11-14

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Includes bibliographical references and index.

MiU

Establishment Of Adventitious Root Cultures Of Echinacea Purpurea For The Production Of Caffeic Acid Derivatives / K.y. Paek, H.n. Murthy And E.j. Hahn -- In Vitro Propagation Of Rauwolfia Serpentina Using Liquid Medium, Assessment Of Genetic Fidelity Of Micropropagated Plants, And Simultaneous Quantitation Of Reserpine, Ajmaline, And Ajmalicine / M.k. Goel [and Others] -- Medicinal Properties, In Vitro Protocols And Secondary Metabolite Analyses Of Scots Pine / H. Haggman [and Others] -- Saussurea Medusa Cell Suspension Cultures For Flavonoid Production / C.z. Liu And P.k. Saxena -- Large-scale In Vitro Multiplication Of Crataeva Nurvala / S.b. Babbar, N. Walia And A. Kaur -- Bilberry In Vitro Protocols And Analyses Of Phenolic Compounds / L. Jaakola [and Others] -- In Vitro Propagation Of Two Tuberous Medicinal Plants: Holostemma Ada-kodien And Ipomoea Mauritiana / S.p. Geetha [and Others] --^ In Vitro Production Of Gymnemic Acid From Gymnema Sylvestre (retz) R. Br. Ex Roemer And Schultes Through Callus Culture Under Abiotic Stress Conditions / A.b. Ali Ahmed, A.s. Rao And M.v. Rao -- Establishment Of Plant Regeneration And Cryopreservation System From Zygotic Embryo-derived Embryogenic Cell Suspension Cultures Of Ranunculus Kazusensis / S.w. Kim And M.j. Oh -- In Vitro Culture And Secondary Metabolite Isolation In Bryophytes / A. Sabovljevic, M. Sabovljevic And N. Jockovic -- Micropropagation And In Vitro Conservation Of Vanilla (vanilla Planifolia Andrews) / M. Divakaran And K.n. Babu -- Protocol For In Vitro Regeneration And Marker Glycoside Assessment In Swertia Chirata Buch-ham / S. Koul [and Others] -- Protocols For Establishment Of An In Vitro Collection Of Medicinal Plants In The Genus Scutellaria / I.b. Cole, F.t. Farooq And S.j. Murch -- Protocols For In Vitro Culture And Phytochemical Analysis Of Phyllanthus Species (euphorbiaceae) / E. Catapan [and Others] --^ In Vitro Clonal Propagation Of Asparagus Racemosus, A High Value Medicinal Plant / S. Saxena And N. Bopana -- Micropropagation Of Penthorum Chinense Through Axillary Buds / J. Yang And Z.s. Peng -- Spontaneous Plant Regeneration And Production Of Secondary Metabolites From Hairy Root Cultures Of Centaurium Erythraea Rafn / A. Subotic [and Others] -- Transgenic Hypericum Perforatum / G. Franklin, M.m. Oliveira And A.c. Dias -- Agrobacterium-mediated Transformation Of Ruta Graveolens L / K. Lievre [and Others] -- Gene Expression Profiling In Taxus Baccata L. Seedlings And Cell Cultures / K. Brun Akova And J. Kosuth -- Catapol Production In Chinese Foxglove (rehmannia Glutinosa Libos.) Hairy Roots Transformed With Agrobacterium Rhizogenes Atcc15834 / S.j. Hwang -- Identification Of Medicinal Plants And Plant Sequences: Multiplexed Mlpa Assay / R.a. Barthelson -- Isolation Of Microsatellites From Catharanthus Roseus (l.) G. Don Using Enriched Libraries / S. Bhatia And B. Shokeen --^ Production Of Cinnamyl Glycosides In Compact Callus Aggregate Cultures Of Rhodiola Rosea Through Biotransformation Of Cinnamyl Alcohol / Z. Gyorgy And A. Hohtola -- Spearmint Plantlet Culture System As A Means To Study Secondary Metabolism / B. Tisserat, M. Berhow And S.f. Vaughn -- Bioreactor Production Of Secondary Metabolites From Cell Cultures Of Periwinkle And Sandalwood / J.v. Valluri -- Camptothecin Production By In Vitro Cultures And Plant Regeneration In Ophiorrhiza Species / T. Asano [and Others] -- Metabolomic Analysis Of Ocotea Odorifera Cell Cultures: A Model Protocol For Acquiring Metabolite Data / M. Maraschin [and Others] -- Production Of 9-methoxycanthin-6-one From Callus Cultures Of (eurycoma Longifolia Jack) Tongkat Ali / M. Maziah And N. Rosli -- Plant Secondary Metabolism In Altered Gravity / L.k. Tuominen, L.h. Levine And M.e. Musgrave -- The Role Of Biotechnology In The Production Of The Anticancer Compound Podophyllotoxin / H. Lata, C.s. Mizuno And R.m. Moraes. Edited By S. Mohan Jain And Praveen K. Saxena. Includes Bibliographical References And Index.

Establishment of adventitious root cultures of Echinacea purpurea for the production of caffeic acid derivatives / K.Y. Paek, H.N. Murthy and E.J. Hahn
In vitro propagation of Rauwolfia serpentina using liquid medium, assessment of genetic fidelity of micropropagated plants, and simultaneous quantitation of reserpine, ajmaline, and ajmalicine / M.K. Goel ... [et al.]
Medicinal properties, in vitro protocols and secondary metabolite analyses of Scots pine / H. Haggman ... [et al.]
Saussurea medusa cell suspension cultures for flavonoid production / C.Z. Liu and P.K. Saxena
Large-scale in vitro multiplication of Crataeva nurvala / S.B. Babbar, N. Walia and A. Kaur
Bilberry in vitro protocols and analyses of phenolic compounds / L. Jaakola ... [et al.]
In vitro propagation of two tuberous medicinal plants: Holostemma ada-kodien and Ipomoea mauritiana / S.P. Geetha ... [et al.]
^
In vitro production of gymnemic acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes through callus culture under abiotic stress conditions / A.B. Ali Ahmed, A.S. Rao and M.V. Rao
Establishment of plant regeneration and cryopreservation system from zygotic embryo-derived embryogenic cell suspension cultures of Ranunculus kazusensis / S.W. Kim and M.J. Oh
In vitro culture and secondary metabolite isolation in bryophytes / A. Sabovljevic, M. Sabovljevic and N. Jockovic
Micropropagation and in vitro conservation of vanilla (Vanilla planifolia Andrews) / M. Divakaran and K.N. Babu
Protocol for in vitro regeneration and marker glycoside assessment in Swertia chirata Buch-Ham / S. Koul ... [et al.]
Protocols for establishment of an in vitro collection of medicinal plants in the genus Scutellaria / I.B. Cole, F.T. Farooq and S.J. Murch
Protocols for in vitro culture and phytochemical analysis of Phyllanthus species (Euphorbiaceae) / E. Catapan ... [et al.]
^
^^
In vitro clonal propagation of Asparagus racemosus, a high value medicinal plant / S. Saxena and N. Bopana
Micropropagation of Penthorum chinense through axillary buds / J. Yang and Z.S. Peng
Spontaneous plant regeneration and production of secondary metabolites from hairy root cultures of Centaurium erythraea Rafn / A. Subotic ... [et al.]
Transgenic Hypericum perforatum / G. Franklin, M.M. Oliveira and A.C. Dias
Agrobacterium-mediated transformation of Ruta graveolens L / K. Lievre ... [et al.]
Gene expression profiling in Taxus baccata L. seedlings and cell cultures / K. Brun akova and J. Kosuth
Catapol production in Chinese foxglove (Rehmannia glutinosa Libos.) hairy roots transformed with Agrobacterium rhizogenes ATCC15834 / S.J. Hwang
Identification of medicinal plants and plant sequences: multiplexed MLPA assay / R.A. Barthelson
Isolation of microsatellites from Catharanthus roseus (L.) G. Don using enriched libraries / S. Bhatia and B. Shokeen
^
^^
Production of cinnamyl glycosides in compact callus aggregate cultures of Rhodiola rosea through biotransformation of cinnamyl alcohol / Z. Gyorgy and A. Hohtola
Spearmint plantlet culture system as a means to study secondary metabolism / B. Tisserat, M. Berhow and S.F. Vaughn
Bioreactor production of secondary metabolites from cell cultures of periwinkle and sandalwood / J.V. Valluri
Camptothecin production by in vitro cultures and plant regeneration in Ophiorrhiza species / T. Asano ... [et al.]
Metabolomic analysis of Ocotea odorifera cell cultures: a model protocol for acquiring metabolite data / M. Maraschin ... [et al.]
Production of 9-methoxycanthin-6-one from callus cultures of (Eurycoma longifolia Jack) Tongkat Ali / M. Maziah and N. Rosli
Plant secondary metabolism in altered gravity / L.K. Tuominen, L.H. Levine and M.E. Musgrave
The role of biotechnology in the production of the anticancer compound podophyllotoxin / H. Lata, C.S. Mizuno and R.M. Moraes.
^^

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants , expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology TM series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.

Front Matter....Pages i-xv
Front Matter....Pages 1-1
Front Matter....Pages 3-16
Front Matter....Pages 17-33
Front Matter....Pages 35-52
Front Matter....Pages 53-59
Front Matter....Pages 61-70
Back Matter....Pages 71-80
....Pages 81-92

2010-01-11