This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, __Patch Clamp Electrophysiology: Methods and Protocols__ aims to be a reference guide for current and future ion channel physiologists.

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scihub/10.1007/978-1-0716-0818-0.pdf

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Humana Press

Methods in Molecular Biology, 2188, 1st edition, New York, NY, 2021

Methods in molecular biology (Clifton, N.J.), New York, NY, 2021

United States, United States of America

1st ed, S.l, 2021

2020

sm84271651

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Front Matter ....Pages i-x
An Introduction to Patch Clamp Recording (Charlotte L. Hill, Gary J. Stephens)....Pages 1-19
Patch Clamp Technology in the Twenty-First Century (Jan Dolzer)....Pages 21-49
Heterologous Expression of Ion Channels in Mammalian Cell Lines (Alistair Mathie, Emma L. Veale, Robyn G. Holden)....Pages 51-65
Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers: Next-Generation Instrumentation for Multiple Recordings in Parallel (Ekaterina Zaitseva, Alison Obergrussberger, Conrad Weichbrodt, Mordjane Boukhet, Frank Bernhard, Christopher Hein et al.)....Pages 67-92
Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology (Kadla R. Rosholm, Kim Boddum, Anders Lindquist)....Pages 93-108
Multielectrode Arrays (Russell Burley, Jenna R. M. Harvey)....Pages 109-132
Utilising Automated Electrophysiological Platforms in Epilepsy Research (Carol J. Milligan, Svenja Pachernegg)....Pages 133-155
Dynamic Clamp on a Windows PC (Lorin S. Milescu, Joël Tabak)....Pages 157-177
Combining Whole-Cell Patch-Clamp Recordings with Single-Cell RNA Sequencing (Kashif Mahfooz, Tommas J. Ellender)....Pages 179-189
Bioluminescence Methodology for Ion Channel Studies (Paul A. Wadsworth, Aditya K. Singh, Nghi Nguyen, Clifford Stephan, Fernanda Laezza)....Pages 191-228
Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons (Francesco Tamagnini)....Pages 229-242
Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method (Timothy W. Church, Matthew G. Gold)....Pages 243-257
In Vivo Patch-Clamp Studies (Yi Zhou, He Li, Zhongju Xiao)....Pages 259-271
In Vivo Optogenetics with Stimulus Calibration (Luke T. Coddington, Joshua T. Dudman)....Pages 273-283
Single or Double Patch-Clamp Recordings In Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics (Jean Simonnet, Louis Richevaux, Desdemona Fricker)....Pages 285-309
Optogenetics and Optical Tools in Automated Patch Clamping (Kim Boddum, Peder Skafte-Pedersen, Jean-Francois Rolland, Sandra Wilson)....Pages 311-330
Back Matter ....Pages 331-333

2020-11-02