Polypeptide materials : Methods and Protocols 🔍
Maxim G. Ryadnov
Springer US;Humana, Methods in Molecular Biology, Methods in Molecular Biology 2208, 1, 2021
英语 [en] · PDF · 10.2MB · 2021 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/scihub/upload/zlib · Save
描述
This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful __Methods in Molecular Biology__ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, __Polypeptide Materials: Methods and Protocols__ serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.
备用文件名
nexusstc/Polypeptide Materials: Methods and Protocols/d3126544d41a18c58106a7201a961924.pdf
备用文件名
lgli/10.1007%2F978-1-0716-0928-6.pdf
备用文件名
lgrsnf/10.1007%2F978-1-0716-0928-6.pdf
备用文件名
scihub/10.1007/978-1-0716-0928-6.pdf
备用文件名
zlib/Engineering/Maxim G. Ryadnov/Polypeptide Materials: Methods and Protocols_11208682.pdf
备选作者
Ryadnov.; MARTON
备用出版商
Humana Press
备用版本
Methods in molecular biology (Clifton, N.J.), New York, NY, 2021
备用版本
Methods in Molecular Biology, 2208, New York, 2020
备用版本
Methods in Molecular Biology 2208, 1st ed., 2021
备用版本
Springer protocols (Series), New York, NY, 2021
备用版本
Springer protocols (Print), New York, 2020
备用版本
United States, United States of America
元数据中的注释
sm83505556
元数据中的注释
producers:
Adobe PDF Library 10.0.1
Adobe PDF Library 10.0.1
元数据中的注释
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备用描述
Front Matter ....Pages i-xiv
A Shortcut to the Synthesis of Peptide Thioesters (Richard Raz, John Offer)....Pages 1-12
SnoopLigase-Mediated Peptide–Peptide Conjugation and Purification (Can M. Buldun, Irsyad N. A. Khairil Anuar, Mark Howarth)....Pages 13-31
Peptide Nanoparticles for Gene Packaging and Intracellular Delivery (Paula Vila-Gómez, James E. Noble, Maxim G. Ryadnov)....Pages 33-48
Synthesis and Application of Peptide–siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung (Jade J. Welch, David A. Dean, Bradley L. Nilsson)....Pages 49-67
FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures (Simon Chi-Chin Shiu, Yusuke Sakai, Julian A. Tanner, Jonathan G. Heddle)....Pages 69-80
Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes (Irene Marzuoli, Franca Fraternali)....Pages 81-100
Functional Peptide Nanocapsules Self-Assembled from β-Annulus Peptides (Hiroshi Inaba, Kazunori Matsuura)....Pages 101-121
Electrostatic Self-Assembly of Protein Cage Arrays (Soumyananda Chakraborti, Antti Korpi, Jonathan G. Heddle, Mauri A. Kostiainen)....Pages 123-133
Design and Generation of Self-Assembling Peptide Virus-like Particles with Intrinsic GPCR Inhibitory Activity (Sergey G. Tarasov, Marzena Dyba, Joshua Yu, Nadya Tarasova)....Pages 135-148
Imaging and 3D Reconstruction of De Novo Peptide Capsids (Emiliana De Santis, Maxim G. Ryadnov)....Pages 149-165
Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra (Abeer Alghamdi, Li Hung C. Chung, Olaf J. Rolinski)....Pages 167-177
Forming Low-Molecular-Weight Hydrogels by Electrochemical Methods (Emily R. Cross, Kate McAulay, Dave J. Adams)....Pages 179-188
In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study (Tristan O. C. Kwan, Rosana Reis, Isabel Moraes)....Pages 189-202
Measurement of Peptide Coating Thickness and Chemical Composition Using XPS (David J. H. Cant, Alexander G. Shard, Caterina Minelli)....Pages 203-224
Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy (Katharine Hammond, Georgina Benn, Isabel Bennett, Edward S. Parsons, Maxim G. Ryadnov, Bart W. Hoogenboom et al.)....Pages 225-235
Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials (Jehangir Cama, Stefano Pagliara)....Pages 237-253
Ultramicrotomy Analysis of Peptide-Treated Cells (Stephanie Rey, Nilofar Faruqui, Maxim G. Ryadnov)....Pages 255-264
Back Matter ....Pages 265-268
A Shortcut to the Synthesis of Peptide Thioesters (Richard Raz, John Offer)....Pages 1-12
SnoopLigase-Mediated Peptide–Peptide Conjugation and Purification (Can M. Buldun, Irsyad N. A. Khairil Anuar, Mark Howarth)....Pages 13-31
Peptide Nanoparticles for Gene Packaging and Intracellular Delivery (Paula Vila-Gómez, James E. Noble, Maxim G. Ryadnov)....Pages 33-48
Synthesis and Application of Peptide–siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung (Jade J. Welch, David A. Dean, Bradley L. Nilsson)....Pages 49-67
FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures (Simon Chi-Chin Shiu, Yusuke Sakai, Julian A. Tanner, Jonathan G. Heddle)....Pages 69-80
Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes (Irene Marzuoli, Franca Fraternali)....Pages 81-100
Functional Peptide Nanocapsules Self-Assembled from β-Annulus Peptides (Hiroshi Inaba, Kazunori Matsuura)....Pages 101-121
Electrostatic Self-Assembly of Protein Cage Arrays (Soumyananda Chakraborti, Antti Korpi, Jonathan G. Heddle, Mauri A. Kostiainen)....Pages 123-133
Design and Generation of Self-Assembling Peptide Virus-like Particles with Intrinsic GPCR Inhibitory Activity (Sergey G. Tarasov, Marzena Dyba, Joshua Yu, Nadya Tarasova)....Pages 135-148
Imaging and 3D Reconstruction of De Novo Peptide Capsids (Emiliana De Santis, Maxim G. Ryadnov)....Pages 149-165
Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra (Abeer Alghamdi, Li Hung C. Chung, Olaf J. Rolinski)....Pages 167-177
Forming Low-Molecular-Weight Hydrogels by Electrochemical Methods (Emily R. Cross, Kate McAulay, Dave J. Adams)....Pages 179-188
In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study (Tristan O. C. Kwan, Rosana Reis, Isabel Moraes)....Pages 189-202
Measurement of Peptide Coating Thickness and Chemical Composition Using XPS (David J. H. Cant, Alexander G. Shard, Caterina Minelli)....Pages 203-224
Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy (Katharine Hammond, Georgina Benn, Isabel Bennett, Edward S. Parsons, Maxim G. Ryadnov, Bart W. Hoogenboom et al.)....Pages 225-235
Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials (Jehangir Cama, Stefano Pagliara)....Pages 237-253
Ultramicrotomy Analysis of Peptide-Treated Cells (Stephanie Rey, Nilofar Faruqui, Maxim G. Ryadnov)....Pages 255-264
Back Matter ....Pages 265-268
开源日期
2020-08-28
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