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lgli/N:\медицина\Bioebooks Anatomia.Color.Atlas.and.Textbook.of.Human.Anatomy.Volume.3.2003.Thieme (156).pdf
Electrophoresis in practice : a guide to methods and applications of DNA and protein separations 🔍
Reiner Westermeier, in collaboration with Sonja Gronau ... [et al.] Wiley-VCH GmbH, 3rd ed., Weinheim, New York, Germany, 2001
英语 [en] · PDF · 11.2MB · 2001 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/zlib · Save
描述
This is your "Laboratory Guide" for successful electrophoretic separations !
Organized in two parts it gives the reader a thorough presentation of the fundamentals followed by a detailed description of 15 of the most common methods currently in use. The Third Edition now includes the latest developments in 2D-electrophoresis as well as an overview of the proteome analysis methodology.
From reviews of the previous editions:
... The rigorous description of each method along with the extensive figures will easily allow the novice to reproduce these methods in the laboratory...
The Analyst
... Perhaps the most important point about the book is that all the recipes provided do actually work. ...
Journal of Laboratory Medicine
... an excellent book which we recommend greatly and which has not to be missed in any laboratory of cellular and molecular biology which respects it self !
Cellular and Molecular Biology
... As a comprehensive guide to the huge variety of electrophoretic methods now available, this is very good value ... The superb ... troubleshooting appendix almost justifies the price on ist own ...
Laboratory Equipment Digest
备用文件名
lgrsnf/N:\медицина\Bioebooks Anatomia.Color.Atlas.and.Textbook.of.Human.Anatomy.Volume.3.2003.Thieme (156).pdf
备用文件名
nexusstc/Electrophoresis in practice: a guide to methods and applications of DNA and protein separations/d0eaa4cf17346bf21198f120e0f79306.pdf
备用文件名
zlib/Biology and other natural sciences/Reiner Westermeier/Electrophoresis in practice: a guide to methods and applications of DNA and protein separations_543595.pdf
备选标题
Proteomics in practice : a laboratory manual of proteome analysis
备选标题
Elektrophorese-Praktikum
备选作者
Westermeier, Reiner, Naven, Tom
备选作者
Reiner Westermeier, Tom Naven
备用出版商
Wiley-Blackwell
备用版本
3. ed, Weinheim [u.a, 2001
备用版本
Weinheim, Germany, 2002
备用版本
Germany, Germany
备用版本
1, PS, 2002
备用版本
3, PS, 2001
元数据中的注释
lg115384
元数据中的注释
{"edition":"3","isbns":["3527303006","3527303545","9783527303007","9783527303540"],"last_page":329,"publisher":"Wiley"}
元数据中的注释
Includes bibliographical references and index.
元数据中的注释
Includes bibliographical references (p. [333]-340) and index.
备用描述
A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries.
The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third section is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further detail.
Intended for all those wishing to go beyond the theoretical aspects of proteome analysis, this book is targeted at research groups within academia and industry, course instructors, research assistants and graduate students.
Special Updates of recipes as well as animations illustrating crucial methodological steps are available on the Web at
备用描述
Pt. I. Proteomics Technology -- Introduction -- Expression Proteomics -- Two-dimensional Electrophoresis -- Spot Handling -- Mass Spectrometry -- Protein Identification By Database Searching -- Methods Of Proteome Analysis -- Pt. Ii. Course Manual -- Step 1. Sample Preparation -- Step 2. Isoelectric Focusing -- Step 3. Sds Polyacrylamide Gel Electrophoresis -- Step 4. Staining Of The Gels -- Step 5. Scanning Of Gels And Image Analysis -- Step 6. Fluorescence Difference Gel Electrophoresis -- Step 7. Spot Excision -- Step 8. Sample Destaining -- Step 9. In-gel Digestion -- Step 10. Microscale Desalting And Concentration Of Sample -- Step 11. Chemical Derivatisation Of The Peptide Digest -- Step 12. Ms Analysis -- Step 13. Calibration Of The Maldi-tof Ms -- Step 14. Preparing For A Database Search -- Step 15. Pmf Database Search Unsuccessful. Reiner Westermeier, Tom Naven. Includes Bibliographical References And Index.
备用描述
Moving boundary electrophoresis: Arne Tiselius (1937) developed the moving boundary technique for the electrophoretic separation of substances, for which, besides his work on adsorption analysis, he received the Nobel prize in 1948.
备用描述
This is a "Laboratory Guide" for electrophoretic separations. Organized in two parts the reader receives a thorough presentation of the fundamentals followed by a detailed description of 15 of the most common methods.
开源日期
2009-10-15
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