MgO recycling in l-lactic acid fermentation and effects of the reusable alkaline neutralizer on Lactobacillus rhamnosus: Fr ... 1
1 Introduction 1
2 Materials and methods 2
2.1 Materials, microorganism and culture media 2
2.2 Cultivation conditions 2
2.3 The MgO recycling process based on magnesium lactate fermentation 2
2.4 Pyrolysis of crude MgCl2 2
2.5 RNA sequencing and transcriptomic analysis 2
2.6 Physico-chemical analysis 3
3 Results and discussion 3
3.1 The feasibility of MgO recycling 3
3.1.1 l-lactic acid extraction 3
3.1.2 The optimization of MgCl2 pyrolysis conditions 3
3.2 Influence of the reusable MgO on l-lactic acid fermentation 4
3.3 Beneficial microenvironment for L. rhamnosus LA-04-1 in magnesium lactate fermentation 4
3.4 The effect of reusable MgO on L. rhamnosus LA-04-1 revealed by transcriptome analysis 5
3.4.1 DEGs in L. rhamnosus LA-04-1 exposed to high concentration of Mg2+ ions 5
3.4.2 Go ontology and functional pathway analyses 6
3.4.2.1 Genes related to amino acid and energy metabolism 7
3.4.2.2 Genes related to carbohydrate metabolism 7
3.4.2.3 Genes related to DNA replication, repair and expression 8
4 Conclusion 8
CRediT authorship contribution statement 8
Declaration of interests 8
Acknowledgements 8
Appendix A Supplementary data 8
References 8

2024-12-23