This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the __Springer Protocols Handbooks__ series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice. Authoritative and practical, __Peptide and Protein Engineering: From Concepts to Biotechnological Applications__ will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering.

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zlib/Engineering/Olga Iranzo, Ana Cecília Roque/Peptide and Protein Engineering: From Concepts to Biotechnological Applications_11208652.pdf

Olga Iranzo; Ana Cecília A Roque

Humana Press

Springer Protocols Handbooks, 1st ed. 2020, New York, NY, 2020

Springer protocols (Series), 1st ed. 2020, New York, NY, 2020

United States, United States of America

Jul 09, 2020

sm82723785

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Source title: Peptide and Protein Engineering: From Concepts to Biotechnological Applications (Springer Protocols Handbooks)

Front Matter ....Pages i-xi
SEA-Mediated Ligation Is Accelerated at Mildly Acidic pH: Application to the Formation of Difficult Peptide Junctions (Marine Cargoët, Vincent Diemer, Laurent Raibaut, Elizabeth Lissy, Benoît Snella, Vangelis Agouridas et al.)....Pages 1-12
The Problem of Aspartimide Formation During Protein Chemical Synthesis Using SEA-Mediated Ligation (Jennifer Bouchenna, Magalie Sénéchal, Hervé Drobecq, Jérôme Vicogne, Oleg Melnyk)....Pages 13-28
Using the Interactive Tool of the Protein Chemical Synthesis Database (Vangelis Agouridas, Oleg Melnyk)....Pages 29-36
Only a “Click” Away: Development of Arginine-Rich Peptide-Based Materials Using Click Chemistry (Mariana Barbosa, Fabíola Costa, Cátia Teixeira, M. Cristina L. Martins, Paula Gomes)....Pages 37-51
Fluoroglycoproteins by Copper-Free Strain-Promoted Azide–Alkyne Cycloaddition (Pedro M. S. D. Cal, Gonçalo J. L. Bernardes, Omar Boutureira)....Pages 53-67
Hybrid Silylated Peptides for the Design of Bio-functionalized Materials (Titouan Montheil, Cécile Echalier, Jean Martinez, Ahmad Mehdi, Gilles Subra)....Pages 69-92
Synthesis of Peptide-Oligoethylene Glycol (OEG) Conjugates for Multivalent Modification of Nanomaterials (Daniel Pulido, Miriam Royo)....Pages 93-123
Phage Display Methodologies (Agathe Urvoas, Philippe Minard, Patrice Soumillion)....Pages 125-151
Whole-Bacterium Ribosome Display Selection for Isolation of Antibacterial Affitins (Ghislaine Béhar, Stanimir Kambarev, Jennifer Jazat, Barbara Mouratou, Frédéric Pecorari)....Pages 153-171
CIS Display: DNA-Based Technology as a Platform for Discovery of Therapeutic Biologics (Ana Margarida Gonçalves Carvalho Dias)....Pages 173-188
Building Scarless Gene Libraries in the Chromosome of Bacteria (Gol Mohammad Dorrazehi, Sebastian Worms, Jason Baby Chirakadavil, Johann Mignolet, Pascal Hols, Patrice Soumillion)....Pages 189-211
Streptavidin (Sav)-Based Artificial Metalloenzymes: Cofactor Design Considerations and Large-Scale Expression of Host Protein Variants (Juliane Klehr, Jingming Zhao, Amanda Santos Kron, Thomas R. Ward, Valentin Köhler)....Pages 213-235
Integrated Bacterial Production and Functional Screening of Expanded Cyclic Peptide Libraries for Identifying Chemical Rescuers of Pathogenic Protein Misfolding and Aggregation (Dafni C. Delivoria, Georgios Skretas)....Pages 237-266
Site-Specific Incorporation of Non-canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli (Uchralbayar Tugel, Meritxell Galindo Casas, Birgit Wiltschi)....Pages 267-281
Back Matter ....Pages 283-289

"This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the Springer Protocols Handbooks series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice. Authoritative and practical, Peptide and Protein Engineering: From Concepts to Biotechnological Applications will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering." -- Prové de l'editor

Springer Protocols Handbooks
Erscheinungsdatum: 09.07.2020

2020-07-10