Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In __High Throughput Protein Expression and Purification: Methods and Protocols__, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, __High Throughput Protein Expression and Purification: Methods and Protocols__ is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.

lgrsnf/MMB/MMB vol.498 Doyle S.A. (ed.) High Throughput Protein Expression and Purification. Methods and Protocols (Humana Press, 2009)(ISBN 1588298795)(322s).pdf

nexusstc/High Throughput Protein Expression and Purification: Methods and Protocols/b543ecd985960ed019511f9909bebf1a.pdf

scihub/10.1007/978-1-59745-196-3.pdf

zlib/Biology and other natural sciences/Jim Koehn, Ian Hunt (auth.), Sharon A. Doyle (eds.)/High Throughput Protein Expression and Purification: Methods and Protocols_657792.pdf

Doyle, Sharon A. (sharon Ann)

edited by Sharon A. Doyle

Methods in molecular biology -- 498, Methods in molecular biology (Clifton, N.J.) -- 498., Totowa, N.J, [London, New Jersey, 2009

Methods in molecular biology (Clifton, N.J.), v. 498, Totowa, N.J. : [London, ©2009

Methods in molecular biology (Clifton, N.J.), Totowa, N.J, cop. 2009

United States, United States of America

2009, 2008

kolxoz-dop

sm20733533

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Includes bibliographical references and index.

Despite Exciting Advances In Genome Sequencing, Isolating A Protein From Its Expression System In Its Native Form Still Presents A Complex Challenge. In 'high Throughput Protein Expression And Purification', Leading Scientists Detail The Most Successful Protocols Currently In Use, Including High Throughput Cloning Schemes. High-throughput Protein Production (http) : A Review Of Enabling Technologies To Expedite Protein Production / Jim Koehn And Ian Hunt -- Designing Experiments For High-throughput Protein Expression / Stephen P. Chambers And Susanne E. Swalley -- Gateway Cloning For Protein Expression / Dominic Esposito, Leslie A. Garvey, And Chacko S. Chakiath -- Flexi Vector Cloning / Paul G. Blommel [and Others] -- The Precise Engineering Of Expression Vectors Using High-throughput In-fusion Pcr Cloning / Nick S. Berrow, David Alderton, And Raymond J. Owens -- The Polymerase Incomplete Primer Extension (pipe) Method Applied To High-throughput Cloning And Site-directed Mutagenesis / Heath E. Klock And Scott A. Lesley -- A Family Of Lic Vectors For High-throughput Cloning And Purification Of Proteins / William H. Eschenfeldt [and Others] -- System 48 High-throughput Cloning And Protein Expression Analysis / James M. Abdullah, Andrzej Joachimiak, And Frank R. Collart -- Automated 96-well Purification Of Hexahistidine-tagged Recombinant Proteins On Magnehis Ni2-particles / Chiann-tso Lin, Priscilla A. Moore, And Vladimir Kery -- E. Coli And Insect Cell Expression, Automated Purification And Quantitative Analysis / Stephenp. Chambers [and Others]. Hexahistidine-tagged Maltose-binding Protein As A Fusion Partner For The Production Of Soluble Recombinant Proteins In Escherichia Coli / Brian P. Austin, Sreedevi Nallamsetty, And David S. Waugh -- Phb-intein-mediated Protein Purification Strategy / Alison R. Gillies, Mahmoud Reza Banki, And David W. Wood -- High-throughput Biotinylation Of Proteins / Brian K. Kay, Sang Thai, And Veronica V. Volgina -- High-throughput Insect Cell Protein Expression Applications / Mirjam Buchs [and Others] -- High-throughput Protein Expression Using Cell-free System / Kalavathy Sitaraman And Deb K. Chatterjee -- The Production Of Glycoproteins By Transient Expression In Mammalian Cells / Joanne E. Nettleship, Nahid Rahman-huq, And Raymond J. Owens -- High-throughput Expression And Detergent Screening Of Integral Membrane Proteins / Said Eshaghi -- Cell-free Expression For Nanolipoprotein Particles : Building A High-throughput Membrane Protein Solubility Platform / Jenny A. Cappuccio [and Others] -- Expression And Purification Of Soluble His6-tagged Tev Protease / Joseph E. Tropea, Scott Cherry, And David S. Waugh -- High-throughput Protein Concentration And Buffer Exchange : Comparison Of Ultrafiltration And Ammonium Sulfate Precipitation / Priscilla A. Moore And Vladimir Kery. Edited By Sharon A. Doyle. Includes Bibliographical References And Index.

High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt
Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley
Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath
Flexi vector cloning / Paul G. Blommel ... [et al.]
The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens
The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley
A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.]
"System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart
Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni2+-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery
E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].
Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh
PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood
High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina
High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.]
High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee
The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens
High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi
Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.]
Expression and purification of soluble His6-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh
High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery.

Front Matter....Pages i-xii
Back Matter....Pages 1-18
....Pages 19-29

Methods in Molecular Biology
Erscheinungsdatum: 20.10.2008

2010-02-18