This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, __Metalloproteins: Methods and Protocols__ aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems.

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Humana Press

Springer New York : Imprint : Humana

Humana Press : Springer

Methods in Molecular Biology, 1st ed. 2019, New York, NY, 2019

Methods in molecular biology (Clifton, N.J.), New York, 2019

Methods in Molecular Biology 1876, 1st ed., 2019

United States, United States of America

Springer protocols, New York, 2018

Oct 13, 2018

sm72376384

producers:
Adobe PDF Library 10.0.1

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Source title: Metalloproteins: Methods and Protocols (Methods in Molecular Biology)

Front Matter ....Pages i-x
Front Matter ....Pages 1-1
Nitrogenases (Nathaniel S. Sickerman, Yilin Hu, Markus W. Ribbe)....Pages 3-24
Enzymatic Systems with Homology to Nitrogenase: Biosynthesis of Bacteriochlorophyll and Coenzyme F430 (Jürgen Moser, Gunhild Layer)....Pages 25-35
Carbon Monoxide Dehydrogenases (Jae-Hun Jeoung, Berta M. Martins, Holger Dobbek)....Pages 37-54
Molybdenum-Containing Enzymes (Dimitri Niks, Russ Hille)....Pages 55-63
Hydrogenases (Nathaniel S. Sickerman, Yilin Hu)....Pages 65-88
Front Matter ....Pages 89-89
Genomic Manipulations of the Diazotroph Azotobacter vinelandii (Patricia C. Dos Santos)....Pages 91-109
Purification of Nitrogenase Proteins (Chi-Chung Lee, Markus W. Ribbe, Yilin Hu)....Pages 111-124
Expression, Purification, and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a,c-Diamide Reductase (Jürgen Moser, Jan Jasper, José Vazquez Ramos, Sven T. Sowa, Gunhild Layer)....Pages 125-140
Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactors (Paul Kaufmann, Chantal Iobbi-Nivol, Silke Leimkühler)....Pages 141-152
Front Matter ....Pages 153-153
Crystallization of Nitrogenase Proteins (Belinda B. Wenke, Renee J. Arias, Thomas Spatzal)....Pages 155-165
X-Ray Crystallography of Carbon Monoxide Dehydrogenases (Jae-Hun Jeoung, Berta M. Martins, Holger Dobbek)....Pages 167-178
X-Ray Absorption Spectroscopy of Metalloproteins (Limei Zhang)....Pages 179-195
Electron Paramagnetic Resonance Spectroscopy of Metalloproteins (Andrew Jasniewski, Yilin Hu, Markus W. Ribbe)....Pages 197-211
Magnetic Circular Dichroism Spectroscopy of Metalloproteins (Brian J. Hales)....Pages 213-225
Front Matter ....Pages 227-227
Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site (Kazuki Tanifuji, Yasuhiro Ohki)....Pages 229-244
Computational Methods for Modeling Metalloproteins (Martin T. Stiebritz, Yilin Hu)....Pages 245-266
Back Matter ....Pages 267-275

Methods in Molecular Biology
Erscheinungsdatum: 13.10.2018

2018-10-14