Molecular Diagnostic PCR Handbook 🔍
Gerrit J. Viljoen, Louis H. Nel, John R. Crowther (auth.)
Springer Nature, 1, 20051206
英语 [en] · PDF · 23.4MB · 2005 · 📘 非小说类图书 · 🚀/lgli/lgrs/nexusstc/scihub/zlib · Save
描述
PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.
备用文件名
lgli/_335628.a9167a4a376324d6bac3a8216da4552e.pdf
备用文件名
lgrsnf/_335628.a9167a4a376324d6bac3a8216da4552e.pdf
备用文件名
scihub/10.1007/1-4020-3404-0.pdf
备用文件名
zlib/Medicine/Gerrit J. Viljoen, Louis H. Nel, John R. Crowther/Molecular Diagnostic PCR Handbook_1056848.pdf
备选作者
by Gerrit J. Viljoen, Louis H. Nel and John R. Crowther
备选作者
John R Crowther; Louis H Nel; Gerrit J Viljoen
备用出版商
Kluwer Academic Publishers
备用出版商
IAEA
备用版本
United States, United States of America
备用版本
Dordrecht, Netherlands, 2005
备用版本
1 edition, December 31, 1899
备用版本
New York, 2005
元数据中的注释
до 2011-08
元数据中的注释
lg617610
元数据中的注释
{"edition":"1","isbns":["1402034032","1402034040","9781402034039","9781402034046"],"last_page":325,"publisher":"Springer"}
元数据中的注释
Handbook.
Copyright by IAEA.
Includes bibliographical references and index.
Copyright by IAEA.
Includes bibliographical references and index.
备用描述
Cover......Page 1
Half-Title......Page 2
Inside Cover......Page 3
Copyright......Page 4
Table of Contents......Page 5
Preface......Page 19
Acknowledgments......Page 20
CHAPTER ONE: BACKGROUND......Page 21
CHAPTER TWO: PCR – THE BASIC REACTION......Page 38
CHAPTER THREE: AMPLICON DETECTION, ANALYSIS AND PCR EVALUATION......Page 68
CHAPTER FOUR: LIMITATIONS AND TROUBLESHOOTING......Page 78
CHAPTER FIVE: SPECIFIC TYPES OF PCR ASSAYS......Page 92
CHAPTER SIX: THE PCR LABORATORY......Page 119
CHAPTER SEVEN: DISEASE DIAGNOSIS USING PCR SPECIFIC PROCEDURES FOR IMPORTANT VETERINARY PATHOGENS......Page 138
APPENDICES......Page 220
APPENDIX A - THERMAL CYCLERS......Page 221
APPENDIX B - BASIC PROTOCOL FOR AGAROSE GEL ELECTROPHORESIIS......Page 226
APPENDIX C - USING NUCLEIC ACID HYBRIDISATION IN ORDER TO VERIFY THE INTEGRITY OF A PCR AMPLICON......Page 228
APPENDIX D - DIGESTION OF DNA WITH RESTRICTION ENDONUCLEASES AND ELECTROPHORESIS OF THE RESTRICTION FRAGMENTS......Page 232
APPENDIX E - DNA SEQUENCE ANALYSIS......Page 235
APPENDIX F - AN EXAMPLE OF A RT-PCR PROTOCOL......Page 240
APPENDIX G(i) - CALIBRATION AND MAINTENANCE OF MECHANICAL PIPETTES......Page 243
APPENDIX G(ii) - STANDARD OPERATING PROCEDURE FOR USING MICROPIPETTES......Page 248
APPENDIX H - STANDARD OPERATING PROCEDURE FOR USING CLOCKS AND TIMERS......Page 250
APPENDIX I - THEORETICAL BACKGROUND TO PH, BUFFERS AND CONCENTRATION OF SOLUTIIONS......Page 252
APPENDIX J - GENERAL BUFFER AND STOCK SOLUTIONS......Page 260
APPENDIX K - GENERAL REAGENTS AND SUPPLIERS......Page 264
APPENDIX L - BIOINFORMATICS FREEWARE, SOFTWARE AND ANALYSIS PROGRAMMES......Page 268
ORIGINAL PAPERS......Page 280
GLOSSARY......Page 294
INDEX......Page 323
Half-Title......Page 2
Inside Cover......Page 3
Copyright......Page 4
Table of Contents......Page 5
Preface......Page 19
Acknowledgments......Page 20
CHAPTER ONE: BACKGROUND......Page 21
CHAPTER TWO: PCR – THE BASIC REACTION......Page 38
CHAPTER THREE: AMPLICON DETECTION, ANALYSIS AND PCR EVALUATION......Page 68
CHAPTER FOUR: LIMITATIONS AND TROUBLESHOOTING......Page 78
CHAPTER FIVE: SPECIFIC TYPES OF PCR ASSAYS......Page 92
CHAPTER SIX: THE PCR LABORATORY......Page 119
CHAPTER SEVEN: DISEASE DIAGNOSIS USING PCR SPECIFIC PROCEDURES FOR IMPORTANT VETERINARY PATHOGENS......Page 138
APPENDICES......Page 220
APPENDIX A - THERMAL CYCLERS......Page 221
APPENDIX B - BASIC PROTOCOL FOR AGAROSE GEL ELECTROPHORESIIS......Page 226
APPENDIX C - USING NUCLEIC ACID HYBRIDISATION IN ORDER TO VERIFY THE INTEGRITY OF A PCR AMPLICON......Page 228
APPENDIX D - DIGESTION OF DNA WITH RESTRICTION ENDONUCLEASES AND ELECTROPHORESIS OF THE RESTRICTION FRAGMENTS......Page 232
APPENDIX E - DNA SEQUENCE ANALYSIS......Page 235
APPENDIX F - AN EXAMPLE OF A RT-PCR PROTOCOL......Page 240
APPENDIX G(i) - CALIBRATION AND MAINTENANCE OF MECHANICAL PIPETTES......Page 243
APPENDIX G(ii) - STANDARD OPERATING PROCEDURE FOR USING MICROPIPETTES......Page 248
APPENDIX H - STANDARD OPERATING PROCEDURE FOR USING CLOCKS AND TIMERS......Page 250
APPENDIX I - THEORETICAL BACKGROUND TO PH, BUFFERS AND CONCENTRATION OF SOLUTIIONS......Page 252
APPENDIX J - GENERAL BUFFER AND STOCK SOLUTIONS......Page 260
APPENDIX K - GENERAL REAGENTS AND SUPPLIERS......Page 264
APPENDIX L - BIOINFORMATICS FREEWARE, SOFTWARE AND ANALYSIS PROGRAMMES......Page 268
ORIGINAL PAPERS......Page 280
GLOSSARY......Page 294
INDEX......Page 323
备用描述
This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented. The difficulties, advantages and disadvantages in PCR applications are explained and placed in context with other test systems. Emphasis is placed on the use of PCR for detection of pathogens with a particular focus on diagnosticians and scientists from the developing world. It is hoped that this book will enable readers from various disciplines and levels of expertise to better judge the merits of PCR and to increase their skills and knowledge in order to assist in a more logical, efficient and assured use of this technology.
备用描述
This Book Gives A Comprehensive Account Of The Practical Aspects Of Pcr And Strong Consideration Is Given To Ensure Its Optimal Use In A Laboratory Environment. This Includes The Theoretical Aspects Related To Pcr, Setting-up Of A Pcr Laboratory And Good Laboratory Practice And Standardised Pcr Protocols To Detect Animal Disease Pathogens. Examples Of Standard Operating Procedures As Used In Individual Specialist Laboratories And An Outline Of Training Materials Necessary For Pcr Technology Transfer Are Presented.--jacket. Background; Pcr -- The Basic Reaction; Amplicon Detection, Analysis And Pcr Evaluation; Limitations And Troubleshooting; Specific Types Of Pcr Assays; The Pcr Laboratory; Disease Diagnosis Using Pcr Specific Procedures For Important Veterinary Pathogens. By Gerrit J. Viljoen, Louis H. Nel And John R. Crowther. Handbook. Copyright By Iaea. Includes Bibliographical References And Index. Mode Of Access: World Wide Web.
开源日期
2011-08-31
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