This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. __Phosphoproteomics Methods and Protocols, Second Edition__ guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, __Phosphoproteomics Methods and Protocols, Second Edition__ aims to ensure successful results in the further study of this vital field.

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Methods in molecular biology, 1355, 2nd ed. 2016, New York, NY, 2016

Methods in Molecular Biology Ser, 2nd ed, New York, Nov. 2015

Methods in Molecular Biology 1355, 2nd ed. 2016, 2015

2. ed, New York, c 2016 [erschienen 2015

United States, United States of America

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Thiol-ene-enabled Detection Of Thiophosphorylation As A Labeling Strategy For Phosphoproteins / Kaelyn E. Wilke And Erin E. Carlson -- Phosphopeptide Detection With Biotin-labeled Phos-tag / Emiko Kinoshita-kikuta, Eiji Kinoshita, And Tohru Koike -- Phosphopeptide Enrichment By Covalent Chromatography After Solid Phase Derivatization Of Protein Digests On Reversed Phase Supports / Heinz Nika, Ruth Hogue Angeletti, And David H. Hawke -- Peptide Labeling Using Isobaric Tagging Reagents For Quantitative Phosphoproteomics / Lei Cheng ... [et Al.] -- Identification Of Direct Kinase Substrates Using Analogue-sensitive Alleles / Daniel A. Rothenberg ... [et Al.] -- Quantitative Analysis Of Tissue Samples By Combining Itraq Isobaric Labeling With Selected/multiple Reaction Monitoring (srm/mrm) / Ryohei Narumi And Takeshi Tomonaga -- Enrichment Strategies In Phosphoproteomics / Alexander Leitner -- Phosphopeptide Enrichment By Immobilized Metal Affinity Chromatography / Tine E. Thingholm And Martin R. Larsen -- Use Of Titanium Dioxide For Selective Enrichment Of Phosphorylated Peptides / Tine E. Thingholm And Martin R. Larsen -- Sequential Elution From Imac (simac) : An Efficient Method For Enrichment And Separation Of Monoand Multi-phosphorylated Peptides / Tine E. Thingholm And Martin R. Larsen -- Improving The Phosphoproteome Coverage For Limited Sample Amounts Using Tio2-simac-hilic (tish) Phosphopeptide Enrichment And Fractionation / Kasper Engholm-keller And Martin R. Larsen -- Offline High Ph Reversed-phase Peptide Fractionation For Deep Phosphoproteome Coverage / Tanveer S. Batth And Jesper V. Olsen -- Phosphopeptide Enrichment Using Various Magnetic Nanocomposites : An Overview / Íris L. Batalha And Ana Cecília A. Roque -- Two Dimensional Gel Electrophoresis-based Plant Phosphoproteomics / Chao Han And Pingfang Yang -- Variable Digestion Strategies For Phosphoproteomics Analysis / Humberto Gonczarowska-jorge ... [et Al.] -- Online Lc-faims-ms/ms For The Analysis Of Phosphorylation In Proteins / Hongyan Zhao, Andrew J. Creese, And Helen J. Cooper -- Simple And Reproducible Sample Preparation For Single-shot Phosphoproteomics With High Sensitivity / Rosa R. Jersie-christensen, Abida Sultan, And Jesper V. Olsen -- Identification Of Direct Kinase Substrates Via Kinase Assay-linked Phosphoproteomics / Liang Xue, Justine V. Arrington, And W. Andy Tao -- Phosphoprotein Detection By High-throughput Flow Cytometry / Johannes Landskron And Kjetil Taskén -- Resources For Assignment Of Phosphorylation Sites On Peptides And Proteins / Vaishnavi Ravikumar, Boris Macek, And Ivan Mijakovic -- From Phosphosites To Kinases / Stephanie Munk ... [et Al.] -- Search Databases And Statistics : Pitfalls And Best Practices In Phosphoproteomics / Jan C. Refsgaard, Stephanie Munk, And Lars J. Jensen -- Systems Analysis For Interpretation Of Phosphoproteomics Data / Stephanie Munk, Jan C. Refsgaard, And Jesper V. Olsen. Edited By Louise Von Stechow. Includes Bibliographical References And Index.

This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. This title guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Front Matter....Pages 3-15
Front Matter....Pages 17-29
Front Matter....Pages 31-50
Front Matter....Pages 51-51
Front Matter....Pages 53-70
Front Matter....Pages 71-84
Back Matter....Pages 85-101
....Pages 103-103

Methods in Molecular Biology
Erscheinungsdatum: 20.11.2015

2015-11-22