This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. __Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations__ pulls together key GMP techniques from laboratories around the world. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Inclusive and authoritative, __Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations__ will be an invaluable resource to both basic and clinical practitioners in stem cell biology.

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Methods in molecular biology (Clifton, N.J.), 1283, New York ; Heidelberg ; Dordrecht, cop. 2015

Methods in molecular biology (Clifton, N.J. Online), 1st ed. 2015, New York, NY, 2015

Methods in molecular biology (Clifton, N.J.), v. 1283, New York, 2015

Methods in molecular biology, 1283, New York, NY, 2015

United States, United States of America

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This Volume Collects A Series Of Protocols Describing The Kinds Of Infrastructures, Training, And Standard Operating Procedures Currently Available To Actualize The Potential Of Stem Cells For Regenerative Therapies. Stem Cells And Good Manufacturing Practices: Methods, Protocols, And Regulations Pulls Together Key Gmp Techniques From Laboratories Around The World. Written In The Highly Successful Methods In Molecular Biology Series Format, Chapters Include Introductions To Their Respective Topics, Lists Of The Necessary Materials, Step-by-step, Readily Reproducible Laboratory Protocols, And Tips On Troubleshooting And Avoiding Known Pitfalls. Inclusive And Authoritative, Stem Cells And Good Manufacturing Practices: Methods, Protocols, And Regulations Will Be An Invaluable Resource To Both Basic And Clinical Practitioners In Stem Cell Biology. Therapeutic Mesenchymal Stromal Cells : Where We Are Headed / Patrick J. Hanley -- Suspension Culture Of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks / Vincent C. Chen And Larry A. Couture -- Scalable Expansion Of Human-induced Pluripotent Stem Cells In Xeno-free Microcarriers / Sara M. Badenes [and 4 Others] -- Derivation Of Gmp-compliant Integration-free Hipscs Using Modified Mrnas / Jens Durruthy Durruthy And Vittorio Sebastiano -- Labeling Pluripotent Stem Cell-derived Neural Progenitors With Iron Oxide Particles For Magnetic Resonance Imaging / Sebastien Sart [and 3 Others] -- Using The Quantum Cell Expansion System For The Automated Expansion Of Clinical-grade Bone Marrow-derived Human Mesenchymal Stromal Cells / Gema Martin-manso And Patrick J. Hanley -- Isolation And Expansion Of Mesenchymal Stromal/stem Cells From Umbilical Cord Under Chemically Defined Conditions / Heba Badraiq, Liani Devito, And Dusko Ilic -- Production Of Good Manufacturing Practice-grade Human Umbilical Cord Blood-derived Mesenchymal Stem Cells For Therapeutic Use / Phuc Van Pham And Ngoc Kim Phan -- Xeno-free Culture Of Human Periodontal Ligament Stem Cells / Oriana Trubiani And Francesca Diomede -- Gmp-compliant Human Adipose Tissue-derived Mesenchymal Stem Cells For Cellular Therapy / Hamid-reza Aghayan, Parisa Goodarzi, And Babak Arjmand -- Cgmp-compliant Transportation Conditions For A Prompt Therapeutic Use Of Marrow Mesenchymal Stromal/stem Cells / Elena Veronesi [and 8 Others] -- Gmp-grade Human Fetal Liver-derived Mesenchymal Stem Cells For Clinical Transplantation / Bagher Larijani, Hamid-reza Aghayan, Parisa Goodarzi, And Babak Arjmand -- Purification Of Human Induced Pluripotent Stem Cell-derived Neural Precursors Using Magnetic Activated Cell Sorting / Goncalo M.c. Rodrigues [and 4 Others] -- Scalable Ex Vivo Expansion Of Human Mesenchymal Stem/stromal Cells In Microcarrier-based Stirred Culture Systems / Joana G. Carmelo, Ana Fernandes-platzgummer, Joaquim M.s. Cabral, And Claudia Lobato Da Silva -- Media Fill For Validation Of A Good Manufacturing Practice-compliant Cell Production Process / Marta Serra, Livia Roseti, And Alessandra Bassi -- Standard Operating Procedure For The Good Manufacturing Practice-compliant Production Of Human Bone Marrow Mesenchymal Stem Cells / Livia Roseti, Marta Serra, And Alessandra Bassi -- Culture Of Human Limbal Epithelial Stem Cells On Tenon's Fibroblast Feeder-layers : A Translational Approach / Gaia Scafetta [and 3 Others] -- Bioreactor Expansion Of Human Mesenchymal Stem Cells According To Gmp Requirements / Christiane L. Elseberg, Denise Salzig, And Peter Czermak. Edited By Kursad Turksen. Includes Bibliographical References And Index.

Front Matter....Pages i-xi
Back Matter....Pages 1-11
....Pages 13-21

Methods in Molecular Biology
Erscheinungsdatum: 14.02.2015

2015-02-17