In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In __Neural Cell Transplantation: Methods and Protocols__, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful __Methods in Molecular BiologyTM__ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, __Neural Cell Transplantation: Methods and Protocols__ provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field.

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zlib/Medicine/Mahendra S. Rao, Mohan C. Vemuri (auth.), David Gordon, Neil J. Scolding (eds.)/Neural cell transplantation: methods and protocols_611179.pdf

Neil Scolding, Neil J. Scolding, David Gordon

edited by Neil J. Scolding and David Gordon

Methods in molecular biology -- v.549, Springer protocols, Methods in molecular biology (Clifton, N.J.) -- v.549., Springer protocols, New York, New York State, 2009

Methods in molecular biology (Clifton, N.J.), v. 549, New York, ©2009

Methods in molecular biology, 1, Totowa, NJ, 2009

United States, United States of America

[Undefined series for scimag]

2009, PT, 2009

torrents.ru tech collections 2009-11-14

sm37214290

sm22122460

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Includes bibliographical references and index.

<p><P>In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation&#58; Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology&#8482; series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls.<p>Cutting-edge and easy to use, Neural Cell Transplantation&#58; Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field.</p> <h3>Doody Review Services</h3> <p><b>Reviewer&#58;</b>Celso Agner, MD, MSc(University of Illinois at Chicago College of Medicine)<BR><b>Description&#58;</b>The field of molecular biology has dramatically changed perspectives on the management of degenerative diseases of the central nervous system. In this book, the authors discuss the current understanding of the management of degenerative diseases of the CNS. <BR><b>Purpose&#58;</b>The main purpose is to provide, in a stepwise fashion, the methods involved in the production of stem cell lines and transplantation into the central nervous system. <BR><b>Audience&#58;</b>Neurologists, neurosurgeons, neurophysiologists, and neuroscientists are the intended audience. The authors are recognized experts in the field.<BR><b>Features&#58;</b>The introductory chapters summarize where we are in terms of stem cells and what aspects should be considered in their preparation prior to transplantation. However, one of the major drawbacks of these first chapters is the lack of a profound analysis of the ethical and religious concerns associated with human stem cells and what alternatives, including omental cells, could be considered to circumvent the limitations of available tissue. Otherwise, the book flows easily and describes the most common research and technical protocols associated with human stem cell handling and separation. It is a well-referenced and illustrated book and worth its value for most labs involved in the research and clinical applications of the technology, in particular for degenerative conditions such as Alzheimer's disease, Parkinson's disease, multiple sclerosis, and others. Another drawback is the brief description of the clinical applications in contrast to the extensive description of the protocols. As a result, the book is a comprehensive reference for neuroscientists, but not so much for clinicians.<BR><b>Assessment&#58;</b>This is a good book for neuroscientists who are directly involved in the separation and production of human stem cells.</p>

Neural transplantation and stem cells / Mahendra S. Rao and Mohan C. Vemuri
Adult stem cells for the treatment of neurological disease / C.M. Rice and N.J. Scolding
Human trials for neurodegenerative disease / Claire M. Kelly, O.J. Handley, and A.E. Rosser
Differentiation of neuroepithelia from human embryonic stem cells / Xiaofeng Xia and Su-Chun Zhang
Derivation of high-purity oligodendroglial progenitors / Maya N. Hatch, Gabriel Nistor, and Hans S. Keirstead
Flow cytometric characterization of neural precursor cells and their progeny / Preethi Eldi and Rodney L. Rietze
Isolation, expansion, and differentiation of adult mammalian neural stem and progenitor cells using the neurosphere assay / Loic P. Deleyrolle and Brent A. Reynolds
Human mesenchymal stem cell culture for neural transplantation / David Gordon and Neil J. Scolding
Umbilical cord blood cells / Jennifer D. Newcomb, Alison E. Willing, and Paul R. Sanberg
Animal models of neurodegenerative diseases / Wendy Phillips ... [et al.]
Animal models of multiple sclerosis / Roberto Furlan, Carmela Cuomo, and Gianvito Martino
Transplantation of oligodendrocyte progenitor cells in animal models of leukodystrophies / Yoichi Kondo and Ian D. Duncan
A rat middle cerebral artery occlusion model and intravenous cellular delivery / Masanori Sasaki, Osamu Honmou, and Jeffery D. Kocsis
MR tracking of stem cells in living recipients / Eva Sykova, Pavla Jendelová, and Vit Herynek
Identifying neural progenitor cells in the adult brain / Stephen Kelly ... [et al.]
Immune ablation followed by autologous hematopoietic stem cell transplantation for the treatment of poor prognosis multiple sclerosis / Harold Atkins and Mark Freedman.

In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology TM series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls

Front Matter....Pages i-xi
Front Matter....Pages 1-1
Front Matter....Pages 3-16
Back Matter....Pages 17-32
....Pages 33-47

2010-01-11