A world-class, concise guide to all things proteomics This book provides a highly authoritative introduction to the promising and fast-advancing field of proteomics, examining the role proteomics plays in the study of biological systems in general and disease in particular. It helps readers understand the structure, function, and interactions of proteins and how this knowledge is used for identifying diseases and developing new drugs. Nawin Mishra, a world-renowned speaker and leader in proteomics studies who has worked with the late Nobel Laureate E. L. Tatum when proteomics was in its infancy, offers an expert perspective on the entire field, including: Easily accessible overview of the principles of proteomics Coverage of real-world, cutting-edge medical applications, including personalized medicine Clear guidelines on how to operate the complex instrumentation involved in proteomics Discussion of the future of proteomics Complete with handy study questions and assuming only a basic understanding of biology, Introduction to Proteomics is an invaluable reference for novices and research scientists alike.Content:
Chapter 1 Historical Perspectives (pages 1–38):
Chapter 2 Proteomics—Relation to Genomics, Bioinformatics (pages 39–60):
Chapter 3 Methodology for Separation and Identification of Proteins and their Interactions (pages 61–102):
Chapter 4 Proteomics of Protein Modifications (pages 103–114):
Chapter 5 Proteomics of Protein–Protein Interactions/Interactomes (pages 115–136):
Chapter 6 Applications of Proteomics I: Proteomics, Human Disease, and Medicine (pages 137–162):
Chapter 7 Proteomics—Future Developments (pages 163–170):

lgrsnf/A:\compressed\10.1002%2F9780470603871.pdf

nexusstc/Introduction to Proteomics: Principles and Applications/7415fc20cd30d2df40bdee76276b2479.pdf

zlib/Medicine/Nawin Mishra(auth.)/Introduction to Proteomics: Principles and Applications_2153430.pdf

Nawin C. Mishra, Günter Blobel, Günter Blobel

Mishra, Nawin C.

Jossey-Bass, Incorporated Publishers

Wiley & Sons, Incorporated, John

WILEY COMPUTING Publisher

Spectrum Publications

Halsted Press

Wiley series in methods of biochemical analysis, Methods of biochemical analysis, Hoboken, N.J, New Jersey, 2010

Methods of biochemical analysis, v. 52, Hoboken, N.J, ©2010

Methods of Biochemical Analysis, 2010 may 04

John Wiley & Sons, Inc., Hoboken, N.J., 2010

United States, United States of America

1, PS, 2010

lg999496

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Includes bibliographical references and index.

(Publisher-supplied data) Table of Content 1. Chapter-1 Historical perspectives 1.1 Introduction to Proteomics 1.2 Proteome and Proteomics A. Proteins as cell?s way of accomplishing specific functions B. Pre-genomic proteomics 1.2.1 Genetics of Proteins A. One gene ?one enzyme theory B. One gene ?many proteins I. RNA Splicing II. RNA Editing References 5. Chapter-5 Proteomics of Protein?Protein interactions /Interactomes 5.1 In vivo analysis of Protein ?Protein Interactions A. Yeast two hybrids Assay B. Phage Display 5.2 In vitro Analysis of Protein Interactions A. Mass Spectrometry (MS) B. Functional Protein Micro array 5.3 In Silico analysis of Protein ?Protein Interactions ? Rosetta Stone and other computational analyses 5.4 Synthetic Genetic method to determine Protein-Protein Interactions 5.5 Interactomes 5.5.1 Prokaryotic interactomes 5.5.2 Eukaryotic interactomes A. Yeast B. Fly C. Worm D. Human E. Plasmodium F. Plants 5.5.3 Interactome during Human Development and Disease 5.6 Evolution and Conservation of Interactomes 5.7 Interactome and complexity of organism 5.8 Interaction of proteins with small molecules References III. RNA Silencing 1.2.2 Molecular Biology of genes and proteins 1.2.3 Protein Chemistry before proteomics References 2. Chapter-2 Proteomics - relation to Genomics, Bioinformatics 2.1 Genomics and Functional Genomics and their utilization in Proteomics 2.2 Bioinformatics and Computational aspects of Proteomics References
3 Methodology 3.1 Separation of proteins- via a multidimensional approach A. Electrophoresis I 2D Gel Electrophoresis II Capillary Electrophoresis B. Liquid Chromatography 3.2 Determination of the protein structure 3.2.1Determination of the Amino acid Sequences
primary structure of proteins
I. Proteomics without Mass Spectrometry
A. Amino acid sequence of a protein from DNA sequence
B. Edman Degradation ? N-terminal amino acid sequence analysis3. Chapter- II. Proteomics based on Mass Spectrometry
A. Mass Spectrometry ? Principle, Historical Background and
Instrumentation
B. Bottom-up and Top-down Mass Spectrometry
C. Bioinformatics and Determination of Amino acid sequence of a protein
None
None
3.2.2 Analysis of Protein 3 Dimensional Structure
A. X-ray Crystallography,
B. Neutron Scattering
C. NMR
None
3.3 Determination of the amount of proteins
A. Quantitation of proteins in 2D gel ( Staining , densitometry)
B. Differential gel analysis ( Dige)
C. Quantitative Mass spectrometric protein ( Isotope labeling , ICAT)
D. Protein Micro Array for large-scale analysis
3.4 Structural and Functional Proteomics
References
None
4. Chapter ? 4 Proteomics of Protein Modifications
None
4.1. Phosphorylation and phosphoproteiomics
4.2. Glycoproteins and glycoproteiomics
4.3. Ubiquitination and ubiquitinomics
4.4. Miscellaneous modifications of proteins
None
6. Chapter -6 Applications
I Application of Proteomics in Medicine: Proteomics, Human Disease and Medicine
6.1 Diseasome
6.2 Medical Proteomics: Proteomics of Human Body Parts
A. Body fluid Proteome
a. Blood /Plasma/Serum Proteome
b. Salivary Proteome
c. Others
B. Liver Proteome
C. Brain Proteome
D. Heart/ cardiovascular Proteome
E. Cancer Proteome
F. Organelle Proteome
G. Proteome of Human Parasites
6.3 Clinical Proteomics
A. Genomics and Proteomics of human diseases
B. Proteomics of Diagnostic markers and Drug Development
C. Proteomics & Personalized Medicine
D. Metabolomics
6.4 Meta Proteomics and Human Health
II. Application of Proteomics in Biotechnology, Industry & others
6.5 Biotechnology & Industry of Drug Production
6.6. Metaproteomics of Microbial Fermentation
a. Metabolite- Production
b. Bioremediation
c. Biomass and Energy production
6.7 Beef Industry
6.8. Bioterrorism & Bio-defense
References
7. Chapter -7 Proteomics - Future
7.1 Technical Scope of Proteomics ? Beyond Protein Identification
7.2 Scientific scope of Proteomics- Control of Epigenesis
7.3 Medical Scope of Proteomics
A. Human Diseases
B. Development of Drugs
C. Personalized Medicine
D. Proteomics and Metabolomics
7.4 Proteomics, Energy Production and bioremediation
7.5 Proteomics and Biodefense

**A world-class, concise guide to all things proteomics** This book provides a highly authoritative introduction to the promising and fast-advancing field of proteomics, examining the role proteomics plays in the study of biological systems in general and disease in particular. It helps readers understand the structure, function, and interactions of proteins and how this knowledge is used for identifying diseases and developing new drugs.
Nawin Mishra, a world-renowned speaker and leader in proteomics studies who has worked with the late Nobel Laureate E. L. Tatum when proteomics was in its infancy, offers an expert perspective on the entire field, including:
* Easily accessible overview of the principles of proteomics
* Coverage of real-world, cutting-edge medical applications, including personalized medicine
* Clear guidelines on how to operate the complex instrumentation involved in proteomics
* Discussion of the future of proteomics
Complete with handy study questions and assuming only a basic understanding of biology, __Introduction to Proteomics__ is an invaluable reference for novices and research scientists alike.Content: Chapter 1 Historical Perspectives (pages 1–38): Chapter 2 Proteomics—Relation to Genomics, Bioinformatics (pages 39–60): Chapter 3 Methodology for Separation and Identification of Proteins and their Interactions (pages 61–102): Chapter 4 Proteomics of Protein Modifications (pages 103–114): Chapter 5 Proteomics of Protein–Protein Interactions/Interactomes (pages 115–136): Chapter 6 Applications of Proteomics I: Proteomics, Human Disease, and Medicine (pages 137–162): Chapter 7 Proteomics—Future Developments (pages 163–170):

"Proteomics provides an introductory insight on proteomics, discussing the basic principles of the field, how to apply specific technologies and instrumentation, and example applications in human health and diseases. With helpful study questions, this textbook presents an easy to grasp and solid overview and understanding of the principles, guidelines, and especially the complex instrumentation operations in proteomics for new students and research scientists. Written by a leader in proteomics studies, Proteomics offers an expert perspective on the field and the future of proteomics"--Provided by publisher.

"Proteomics provides an introductory insight on proteomics, discussing the basic principles of the field, how to apply specific technologies and instrumentation, and example applications in human health and diseases. With helpful study questions, this textbook presents an easy to grasp and solid overview and understanding of the principles, guidelines, and especially the complex instrumentation operations in proteomics for new students and research scientists. Written by a leader in proteomics studies, Proteomics offers an expert perspective on the field and the future of proteomics"--Résumé de l'éditeur

2013-08-01