Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.
Erscheinungsdatum: 31.12.2003

Dykstra, Michael J., Reuss, Laura E.

Da Capo Press, Incorporated

Hachette Books

Springer US

Hachette GO

United States, United States of America

Dordrecht, 2003

Includes bibliographical references and index.

<p>Dykstra and Reuss, both of North Carolina State University, offer an updated blend of technical approaches and didactic information found in Dykstra's <i>Biological Electron Microscopy: Theory, Techniques, and Troubleshooting</i>, 1992, and <i>A Manual of Applied Techniques for Biological Electron Microscopy</i>, 1993, in this combined text and lab manual intended for a one-semester course that covers basic approaches to light, transmission electron, and scanning electron microscopy. This edition covers the many innovations in instrumentation and digital control and imaging occurring in the field over the past 10 years. B&w images from the first edition have been retained, and lists of Web sites, organizations, and suppliers have been updated. Annotation &copy;2004 Book News, Inc., Portland, OR</p> <h3>Booknews</h3> <p>Many black and white photographs illustrate this one-semester textbook of electron microscopy. Topics are all of a practical nature and include specimen preparation, ultramicrotomy, support films, transmission electron microscopy, vacuum systems, staining methods for semithins and ultrathins, photography, and shadowing and staining. References are given at the end of each chapter and appendices are included on laboratory safety, literature sources, and equipment and supplies. Annotation c. Book News, Inc., Portland, OR (booknews.com)</p>

xviii, 534 pages ; 26 cm
Includes bibliographical references and index

2024-07-01