Yeast Protocols contains many key techniques for studying the biology of yeasts at both the cellular and molecular levels. Working primarily from Saccharomyces cerevisiae, the expert contributors explain step-by-step how to successfully isolate, identify, and culture yeasts; the secrets of meiotic mapping; how to use PFGE in karyotyping and gene localization; the methods for purification and analysis of various cell components; and the construction and exploitation of genomic DNA clone banks. They also cover the latest methods for chromosome engineering, insertional mutagenesis by Ty elements, mRNA abundance and half-life measurements, the use of reporter gene systems, genotoxicity testing, and more. Yeast Protocols follows the widely applauded Humana Methods in Molecular Biology style: brief introductions putting the particular method in context, comprehensive lists of materials, cookbook style instructions, and troubleshooting notes to avoid common pitfalls and solve problems. The techniques can be used with confidence and success by both inexperienced newcomers and established researchers.

lgrsnf/MMB/MMB vol.053 Evans I.H. (ed.) Yeast Protocols.. Methods in Cell and Molecular Biology (Humana Press, 1996)(ISBN 0896033198)(C)(T)(402s).djvu

nexusstc/Yeast Protocols: Methods in Cell and Molecular Biology/26f3511052e9aa42edb91c6cf86e5ba8.djvu

zlib/Biology and other natural sciences/Ivor H. Evans/Yeast Protocols: Methods in Cell and Molecular Biology_657375.djvu

edited by Ivor H. Evans

Methods in molecular biology ;, v. 53, Methods in molecular biology (Clifton, N.J.) ;, v. 53., Totowa, N.J, New Jersey, 1996

Methods in molecular biology, 313, Totowa, 2006

Spiral edition, Totowa, New Jersey, March 1996

United States, United States of America

1, 1996

kolxoz-dop

lg229825

{"isbns":["0896033198","9780896033191"],"last_page":422,"publisher":"Humana Press","series":"Methods in Molecular Biology"}

Includes bibliographical references and index.

Isolation And Identification Of Yeasts From Natural Habitats / John F. Spencer, Dorothy M. Spencer -- Maintenance And Culture Of Yeasts / John F. Spencer, Dorothy M. Spencer -- Mutagenesis In Yeast / John F. Spencer, Dorothy M. Spencer -- Rare-mating And Cytoduction In Saccharomyces Cerevisiae / John F. Spencer, Dorothy M. Spencer -- Protoplast Fusion In Saccharomyces Cerevisiae / Brendan P. Curran, Virginia C. Bugeja -- Meiotic Analysis / John F. Spencer, Dorothy M. Spencer -- Ascus Dissection / Carl Saunders-singer -- Karyotyping Of Yeast Strains By Pulsed-field Gel Electrophoresis / John R. Johnston -- Chromosomal Localization Of Genes Through Pulsed-field Gel Electrophoresis Techniques / Graham R. Bignell, Ivor H. Evans -- Isolation, Purification, And Analysis Of Nuclear Dna In Yeast Taxonomy / Ann Vaughan-martini, Alessandro Martini -- Isolation Of Yeast Dna / Peter Piper -- Isolation Of Mitochondrial Dna / Graham R. Bignell, Angela R. Miller, Ivor H. Evans --^ Isolation Of Yeast Plasma Membranes / Barry Panaretou, Peter Piper -- The Extraction And Analysis Of Sterols From Yeast / Michael A. Quail, Steven L. Kelly -- Peroxisome Isolation / Ben Distel, Inge Leij, Wilko Kos -- Transformation Of Lithium-treated Yeast Cells And The Selection Of Auxotrophic And Dominant Markers / Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield -- Biolistic Transformation Of Yeasts / Stephen A. Johnston, Michael J. Devit -- Genomic Yeast Dna Clone Banks: Construction And Gene Isolation / Graham R. Bignell, Ivor H. Evans -- Yeast Colony Hybridization / Matthew John Kleinman -- Determination Of Plasmid Copy Number In Yeast / Bernadette E. Jordan, Robert C. Mount, Christopher Hadfield -- Determination Of Chromosome Ploidy In Yeast / Christopher Hadfield, Bernadette E. Jordan, Robert C. Mount -- Chromosome Engineering In Yeast With A Site-specific Recombination System From A Heterologous Yeast Plasmid / Yasuji Oshima, Hiroyuki Araki, Hiroaki Matsuzaki --^ Insertional Mutagenesis By Ty Elements In Saccharomyces Cerevisiae / David J. Garfinkel -- Reporter Gene Systems For Assaying Gene Expression In Yeast / Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield -- Preparation And Use Of Yeast Cell-free Translation Lysate / Alan D. Hartley, Manuel A. Santos, David R. Colthurst, Michael F. Tuite -- Electrophoretic Analysis Of Yeast Proteins / Christopher M. Grant, Ian T. Fitch, Michael F. Tuite -- Preparation Of Total Rna / Alistair J. Brown -- Mrna Abundance And Half-life Measurements / Alistair J. Brown, Francis A. Sagliocco -- Polysome Analysis / Francis A. Sagliocco, Paul A. Moore, Alistair J. Brown -- Induction Of Heat Shock Proteins And Thermotolerance / Peter Piper -- Rhodamine B Assay For Estimating Activity Of Killer Toxins Permeabilizing Cytoplasmic Membranes / Vladimír Vondrejs, Zdena Palková --^ Nystatin-rhodamine B Assay For Estimating Activity Of Killer Toxin From Kluyveromyces Lactis / Zdena Palková, Vladimír Vondrejs -- Application Of Killer Toxins In Stepwise Selection Of Hybrids And Cybrids Obtained By Induced Protoplast Fusion / Vladimír Vondrejs, Zdena Palková, Zuzana Zemanová -- Killer Plaque Technique For Selecting Hybrids And Cybrids Obtained By Induced Protoplast Fusion / Zdena Palková, Vladimír Vondrejs -- Genotoxicity Testing In Schizosaccharomyces Pombe / Pamela Mcathey -- Purification And Quantification Of Saccharomyces Cerevisiae Cytochrome P450 / Ian Stansfield, Steven L. Kelly -- Calorimetry Of Whole Yeast Cells / Linda J. Ashby, Anthony E. Beezer -- Light Microscopy Methods / Eva Streiblová, Jiří Hašek -- Fluorescence Microscopy Methods / Jiří Hašek, Eva Streiblová -- Immunoelectron Microscopy / Ever-jan Tuinen. Edited By Ivor H. Evans. Includes Bibliographical References And Index.

2010-02-18