Biopolymers at Interfaces......Page 2
Preface......Page 8
Contents......Page 9
Contributors......Page 12
MACROMOLECULES ARE SOFT PARTICLES......Page 16
Table of Contents......Page 0
ADSORPTION AND PARTICLE DEFORMATION......Page 18
FORMATION OF AN ADSORBED LAYER......Page 19
THE HOMOGENEOUS LAYER IN EQUILIBRIUM......Page 20
ADSORPTION AND DESORPTION RATES......Page 23
STRUCTURE OF ADSORBED LAYERS......Page 24
POLYMER MIXTURES: PREFERENCE AND EXCHANGE......Page 25
SPREADING: EXPERIMENTAL EVIDENCE......Page 26
POLYELECTROLYTES AND POLYAMPHOLYTES......Page 29
REFERENCES......Page 33
INTRODUCTION......Page 36
TRENDS IN THE ADSORPTION BEHAVIOR OF UNCHARGED AND CHARGED FLEXIBLE POLYMERS......Page 37
STRUCTURE AND STABILITY OF GLOBULAR PROTEINS IN AQUEOUS SOLUTION......Page 40
Coulomb Interaction......Page 41
Lifshitz – van der Waals Interactions......Page 42
Bond Lengths and Bond Angles......Page 43
ADSORPTION OF PROTEINS FROM AQUEOUS SOLUTION ONTO SOLID SURFACES......Page 45
Redistribution of Charged Groups Resulting from Overlapping Electrical Double Layers......Page 46
Dispersion Interaction......Page 51
Hydration Changes......Page 52
Rearrangements in the Protein Structure......Page 53
CONCLUSIONS......Page 54
REFERENCES......Page 55
INTRODUCTION......Page 59
Dynamic Approach......Page 60
Amino Acids......Page 61
Linear Peptides......Page 70
Nucleic Acid Bases......Page 76
Nucleotides......Page 79
CONCLUSIONS AND FUTURE PROSPECTS......Page 82
REFERENCES......Page 83
INTRODUCTION......Page 85
Electrostatics......Page 88
van der Waals Interactions......Page 92
Solvation Forces......Page 95
RELATION TO BULK ADSORPTION MEASUREMENTS......Page 96
Protein – Surface Interactions......Page 97
Protein – Protein Interactions......Page 100
REFERENCES......Page 104
INTRODUCTION......Page 109
INTERFACIAL BEHAVIOR OF NATURALLY OCCURRING PROTEIN VARIANTS......Page 110
INTERFACIAL BEHAVIOR OF STABILITY MUTANTS......Page 111
INTERFACIAL BEHAVIOR OF INTERACTION MUTANTS......Page 116
INTERFACIAL BEHAVIOR OF ASSOCIATION MUTANTS......Page 124
ACKNOWLEDGMENTS......Page 125
REFERENCES......Page 126
INTRODUCTION......Page 128
Preparation of Antibodies and Their Fragments......Page 132
Chemical Modification of Antibodies and Their Fragments......Page 133
Immobilization of Antibodies......Page 137
Immunoassays......Page 140
Orientation of Immobilized Antibodies......Page 145
Activity of Immobilized Antibodies......Page 146
Packing and Orientation of Acid-Pretreated Antibodies Immobilized by Physical Adsorption......Page 162
Applications of Immobilized Antibodies......Page 168
CONCLUSIONS......Page 169
REFERENCES......Page 170
INTRODUCTION......Page 177
van der Waals Forces......Page 179
Electrostatic Double-Layer Forces......Page 181
Repulsive Short-Range Forces......Page 182
Hydrogen Bonds......Page 183
Interactions Due to Adsorbed Uncharged Polymer Layers......Page 184
METHODS......Page 185
Sugar-Based Surfactants......Page 188
Glycolipids......Page 192
Polysaccharides......Page 195
Glycoproteins......Page 201
SUMMARY......Page 204
REFERENCES......Page 205
INTRODUCTION — THE KINETIC IMPERATIVE......Page 210
FUNDAMENTAL CHARACTERISTICS OF AN ADSORPTION PROCESS......Page 211
EXPERIMENTAL PREREQUISITES......Page 215
Measuring the Rate of Accumulation......Page 216
Obtaining Complementary Information......Page 217
The Memory Function......Page 218
The Adsorption Rate Coefficient......Page 219
Transport Phenomena......Page 220
Exclusion Zones......Page 221
Postadsorption Changes and Desorption......Page 223
COLLECTIVE EFFECTS......Page 224
Adsorption to a Continuum......Page 225
Adsorption to Discrete Receptor Sites......Page 226
PERSPECTIVES......Page 227
LIST OF PRINCIPAL SYMBOLS......Page 228
REFERENCES......Page 229
INTRODUCTION......Page 232
Two-Dimensional Order......Page 233
Relevance to Experimentation: Effect of Protein Mobility on Scanning Probe Microscopy......Page 234
Basic Insight into Adsorbed Layer Heterogeneity......Page 235
INDIRECT EVIDENCE FOR ADSORBED PROTEIN MOBILITY......Page 236
Fluorescence Recovery After Photobleaching......Page 238
Other Techniques......Page 247
WHICH DIFFUSION COEFFICIENT IS MEASURED?......Page 248
DIRECT MEASUREMENTS OF SURFACE DIFFUSION......Page 250
Lateral Interactions: Effect of Coverage......Page 252
Origin of the Immobile Fraction......Page 255
SURFACE DIFFUSION MECHANISM......Page 257
Comparison of Adsorbed Proteins......Page 259
FUTURE RESEARCH......Page 263
REFERENCES......Page 264
INTRODUCTION......Page 269
Insulin......Page 271
b-Lactoglobulin......Page 281
ADSORPTION OF SELF-ASSOCIATING PROTEINS WITH UNORDERED STRUCTURE......Page 284
ADSORPTION IN RELATION TO (HEAT) AGGREGATION OF PROTEINS......Page 291
ACKNOWLEDGMENT......Page 296
REFERENCES......Page 297
INTRODUCTION......Page 305
EXPERIMENTAL TECHNIQUES FOR STUDIES OF COMPETITIVE AND SEQUENTIAL ADSORPTION REACTIONS......Page 307
STUDIES OF THE REACTION MECHANISM OF EXCHANGE: GENERAL RESULTS......Page 311
PERSPECTIVES AND CONCLUDING REMARKS......Page 320
REFERENCES......Page 323
GENERAL FEATURES OF THE INTERACTIONS BETWEEN PROTEINS AND SURFACTANTS AT SOLID INTERFACES......Page 331
Removal of Proteins from Solid Interfaces by Surfactants......Page 332
Competitive Adsorption of Proteins and Surfactants......Page 334
Surfactant......Page 335
Protein......Page 339
Surface......Page 341
Cleaning Food Processing Equipment......Page 344
Dental Applications......Page 345
Biochemical Applications......Page 346
CONCLUDING REMARKS......Page 347
REFERENCES......Page 348
INTRODUCTION......Page 354
Interactions of Proteins and Polyelectrolytes in Solution......Page 356
Adsorption of Proteins on Polyelectrolyte Multilayers......Page 357
Structure of Proteins Adsorbed on or Embedded in Polyelectrolyte Multilayers......Page 360
Diffusion of Proteins on and in Polyelectrolyte Multilayers......Page 362
Construction of Polyelectrolyte Multilayers with Natural Polyelectrolytes......Page 363
Interaction of Polyelectrolyte Multilayers with Cells......Page 365
BIOMINERALIZATION INDUCTION BY POLYELECTROLYTE MULTILAYER FILMS......Page 370
REFERENCES......Page 372
INTRODUCTION: ALTERNATE LAYER-BY-LAYER ADSORPTION AS A NOVEL METHODOLOGY FOR PROTEIN ORGANIZATION......Page 376
PRACTICAL PROCEDURE AND ADVANTAGES......Page 378
EVALUATION OF ASSEMBLING PROCESS......Page 380
PLANAR FILM – TYPE ENZYME NANOREACTOR......Page 385
PROTEIN ASSEMBLY ON MICROTEMPLATE......Page 390
ENZYME-CONTAINING POLYION CAPSULE......Page 394
FUTURE PERSPECTIVE......Page 396
REFERENCES......Page 397
BIOCHEMICAL BASIS OF THE BIOLOGICAL ACTIVITY OF ADSORBED PROTEINS......Page 401
Affinity of the Adhesion Proteins......Page 403
Types of Nonfouling Materials......Page 404
Poly(Ethylene Oxide) – Like Nonfouling Materials......Page 405
Antibody Binding Studies......Page 410
Cell Interactions......Page 413
REFERENCES......Page 416
Model Membranes......Page 422
Monolayer Characterization: Surface Pressure and Surface Potential......Page 424
Protein Interfacial Activity and Surface Tension......Page 425
Light Reflection at the Air – Water Interface......Page 426
Electrostatic Binding of Proteins to Monolayers......Page 427
Amphiphile Miscibility, Phases, and Protein Adsorption......Page 428
Enhanced Protein Adsorption to Mixed Monolayers: Ferritin Binding to Mixed Cationic/Neutral Monolayers......Page 429
Amphiphile Miscibility and Mixing Diagrams......Page 431
PEO Surface Density Versus PEO Brush Length......Page 434
In Situ SPR Measurements of PS-PEO Monolayer Protein Interactions......Page 436
Protein – PS-PEO Monolayer Interactions Sensed by the Surface Pressure Changes......Page 438
REFERENCES......Page 439
Interaction of Protein Molecules with Light......Page 442
Surface Versus Bulk Sensitivity......Page 443
Proteins’ Private and Public Lives......Page 446
Local Versus Global Spectroscopic Probes of Protein Structure and Dynamics......Page 447
Protein Fluorescence......Page 449
Total Internal Reflection Fluorescence Spectroscopy......Page 452
INFRARED SPECTROSCOPY AND CIRCULAR DICHROISM AS GLOBAL PROBES OF PROTEIN MOLECULES......Page 458
Infrared Spectroscopy......Page 459
Analysis of Protein Conformation from IR Spectra......Page 462
Circular Dichroism......Page 464
REMAINING PROBLEMS AND FUTURE OUTLOOK......Page 466
REFERENCES......Page 467
INTRODUCTION......Page 474
Sampling Modes......Page 475
Basics of Attenuated Total Internal Reflection......Page 476
Quantification of Adsorbed Proteins......Page 477
CAN H2O BE SUBTRACTED FROM PROTEIN SPECTRA?......Page 480
Relationship to X-Ray Crystallography......Page 483
Quantitation of Secondary Structure......Page 484
FTIR and Blood Proteins......Page 485
FTIR and Orientation......Page 486
FTIR AND MULTIPLE PROTEIN SOLUTIONS......Page 487
ATOMIC FORCE MICROSCOPY AND SURFACES......Page 488
SURFACE MODIFICATION STRATEGIES......Page 490
Self-Assembled Monolayers for Surface Modification......Page 491
SAMs in Biomaterials Research......Page 492
RGD Peptides on Surfaces......Page 493
CONCLUSIONS......Page 495
REFERENCES......Page 496
CONFORMATIONAL CHANGES OF PROTEINS AT SOLID SURFACES......Page 504
THE MOLTEN GLOBULE STATE......Page 505
Bovine Serum Albumin......Page 506
Human Carbonic Anhydrase II......Page 507
Circular Dichroism......Page 508
Fluorescence......Page 509
CONFORMATIONAL CHANGES OF STABILITY MUTANTS OF BACTERIOPHAGE T4 LYSOZYME ADSORBED ONTO COLLOIDAL SILICA PARTICLES......Page 510
TRUNCATED VARIANTS OF HCAII ADSORBED TO NANOPARTICLES OBTAIN A MOLTEN GLOBULE-LIKE STATE......Page 513
ACKNOWLEDGMENTS......Page 518
REFERENCES......Page 519
INTRODUCTION — PROTEINS AT INTERFACES......Page 523
TRANSITION ENTHALPIES AS STABILITY CHARACTERISTICS......Page 524
THE DSC MEASUREMENT OF PROTEINS IN SOLUTION......Page 525
HEAT CAPACITY INCREASES DUE TO THE INCREASE IN HYDROPHOBICITY DURING DENATURATION......Page 528
PROTEINS AT INTERFACES AS SEEN BY DSC......Page 530
Substrate Effects on Conformation......Page 531
Interaction with Separation Matrices......Page 533
Stabilization Through Formation of Solid Phase Affinity Complexes......Page 536
Antibodies as Affinity Anchors......Page 538
Direct Adsorption versus Tethered Attachment......Page 539
ACKNOWLEDGMENTS......Page 541
REFERENCES......Page 542
METHODOLOGY......Page 545
Ellipsometry......Page 546
Reflectometry......Page 549
Surfaces......Page 551
Surface Modifications......Page 555
Comparison with Other Methods......Page 559
Multilayers......Page 561
Examples of Adsorbed Layer Structure in Biopolymer Systems......Page 562
INVESTIGATIONS OF OPTICAL PROPERTIES OF ADSORBED PROTEINS......Page 564
Mass Transport......Page 565
Affinity Measurements......Page 566
Adsorbed Layer Formation......Page 567
Multicomponent Systems......Page 571
Interfacial Degradation and Synthesis......Page 577
REFERENCES......Page 580
INTRODUCTION......Page 589
SURFACE PLASMON SPECTROSCOPY AND SURFACE PLASMON FIELD-ENHANCED FLUORESCENCE SPECTROSCOPY......Page 590
Monitoring Protein Adsorption/Desorption Processes by SPR......Page 591
Simultaneous SPR and SPFS Studies......Page 593
MINIMIZING NONSPECIFIC ADSORPTION TO POLYMER-MODIFIED SURFACES......Page 595
SURFACE RECONSTITUTION OF CYTOCHROME b562......Page 599
INTERACTION OF SINGLE STRAND BINDING PROTEINS WITH SURFACE-ATTACHED OLIGONUCLEOTIDES......Page 602
RECOGNITION OF STEROL MOLECULES IN TETHERED MEMBRANES......Page 607
CONCLUSIONS AND OUTLOOK......Page 611
REFERENCES......Page 612
INTRODUCTION......Page 614
NEUTRON REFLECTION AND DATA ANALYSIS......Page 615
EXCHANGE OF LABILE HYDROGENS......Page 617
ADSORPTION AT THE HYDROPHILIC SILICON OXIDE – WATER INTERFACE......Page 621
ADSORPTION AT THE HYDROPHOBIC SOLID – WATER INTERFACE......Page 626
ADSORPTION AT THE C15OH – WATER INTERFACE......Page 631
ADSORPTION AT THE PC MONOLAYER – SOLUTION INTERFACE......Page 639
SUMMARY......Page 642
REFERENCES......Page 644
INTRODUCTION......Page 646
General Considerations......Page 648
Characterization of Protein Adsorption......Page 649
Limitations of the Technique......Page 653
General Considerations......Page 654
Static ToF-SIMS Analysis of Peptides and Proteins......Page 655
Challenges and Advances in Static ToF-SIMS Analysis of Proteins......Page 656
Static ToF-SIMS Chemical State Imaging......Page 659
Emerging Technologies and Applications......Page 660
General Considerations......Page 661
Characterization of Adsorbed Protein Films......Page 663
Study of Competitive Protein Adsorption and Displacement......Page 666
Present Limitations......Page 667
CONCLUSIONS......Page 669
REFERENCES......Page 670
Synthetic Membranes and Biotechnology......Page 675
Analyzing the Problem......Page 677
Overview of Phenomena......Page 678
Protein Adsorption......Page 679
Protein Deposition......Page 685
Properties of Protein Deposits......Page 690
Protein – Protein Interactions......Page 692
Biological Molecule Interactions (Nonproteins)......Page 693
Protein – Polymer Interactions......Page 694
Control of Solution Conditions......Page 700
Use of Surface Modification Techniques......Page 701
REFERENCES......Page 707
INTRODUCTION......Page 714
Micelles......Page 715
Liposomes......Page 716
Others......Page 717
Methodology......Page 718
Effects of Wettability......Page 719
Effects of Charge......Page 720
Mixed Phospholipid Systems......Page 722
Effects of Lipid Fluidity......Page 723
Adsorption from Serum and Plasma......Page 724
Complement Activation......Page 725
Controlled Circulation Time and Tissue Distribution Through Use of PEG-Containing Carriers......Page 726
Mechanisms of Protein Rejection......Page 727
Different Types of PEG Modification......Page 729
Effects of Chain Length......Page 730
Effects of Chain Density......Page 731
Effects of Anchoring......Page 733
Mechanisms and Effects of Solvency......Page 734
CONCLUDING REMARKS......Page 735
REFERENCES......Page 736
INTRODUCTION......Page 743
PRINCIPLES OF SOLID-PHASE DIAGNOSTICS......Page 744
PRINCIPLES OF THERAPEUTIC APHERESIS......Page 745
Protein Adsorption......Page 747
Poly(Ethylene Glycol) – Grafted Surfaces......Page 748
Immobilization of Proteins to Poly(Ethylene Glycol) – Grafted Surfaces......Page 758
Site-Specific Immobilization......Page 761
SOLID-PHASE DIAGNOSTICS ON HYDROPHILIZED PLATES......Page 762
NONSPECIFIC THERAPEUTIC APHERESIS: REMOVAL OF LOW-MOLECULAR-WEIGHT PROTEINS......Page 764
SPECIFIC THERAPEUTIC APHERESIS: REMOVAL OF HIGH-MOLECULAR-WEIGHT PROTEINS ON HYDROPHILIZED CARTRIDGES......Page 765
Removal of Antibodies of a Defined Specificity......Page 766
CONCLUSIONS......Page 768
REFERENCES......Page 769
INTRODUCTION......Page 774
Surface Area – to – Bulk Volume Ratio......Page 775
Diffusion Length......Page 777
Electroosmotic Flow......Page 778
Other Methods for Fluid Control......Page 781
SEPARATION OF PROTEINS USING CAPILLARY ELECTROPHORESIS......Page 782
IMMUNOASSAYS......Page 784
Tryptic Digestion......Page 793
MICROARRAYS......Page 798
CONCLUSIONS......Page 804
REFERENCES......Page 805
INTRODUCTION......Page 812
SALIVARY PROTEINS......Page 813
Globular......Page 815
Flexible......Page 816
Protein Association Phenomena and Structure Formation in Saliva......Page 818
ORAL INTERFACES......Page 819
PELLICLE......Page 821
Adsorption from Salivary Solutions — Physicochemical Properties......Page 823
Behavior of Isolated Protein Fractions......Page 831
Aspects of Composition and Development......Page 835
PLAQUE FORMATION......Page 838
ROUTES FOR MODULATION OF PLAQUE GROWTH......Page 841
SALIVARY SUBSTITUTES......Page 843
REFERENCES......Page 844
INTRODUCTION......Page 857
b-Casein......Page 859
Adsorbed b-Lactoglobulin......Page 865
Surface Studies......Page 866
KINETICS OF ADSORPTION OF PROTEINS AT FLUID INTERFACES......Page 867
Protein Adsorption at the Oil – Water Interface......Page 869
Protein Adsorption at the Air – Water Interface......Page 872
Adsorbed Protein Films at the Oil – Water Interface......Page 875
Adsorbed and Spread Protein Films at the Air – Water Interface......Page 876
Long-Term Relaxation Phenomena of Spread Protein Films at the Air – Water Interface......Page 878
Surface Dilatational Characteristics of Spread Protein Films at the Air – Water Interface......Page 880
GELATION OF ADSORBED PROTEIN FILMS AT THE OIL – WATER INTERFACE......Page 882
Interactions Between Protein-Coated Surfaces......Page 884
Comparison of as1-Casein and b-Casein Behavior......Page 885
COMPETITIVE DISPLACEMENT......Page 886
Physical Methods......Page 887
Competitive Adsorption and Displacement in Protein – Lipid Mixed Systems......Page 889
CONCLUDING REMARKS......Page 894
REFERENCES......Page 895

lgli/2204.Biopolymers at Interfaces Second Edition.pdf

lgrsnf/2204.Biopolymers at Interfaces Second Edition.pdf

zlib/Medicine/Martin Malmsten/Biopolymers at Interfaces, Second Edition_599820.pdf

surfactant science series volume 110 BIOPOLYMERS AT INTERFACES

Biopolymers at Interfaces (SURFACTANT SCIENCE SERIES)

Biopolymers at interfaces: edited by Martin Malmsten

Martin Malmsten

Marcel Dekker Incorporated

Surfactant science series ;, v. 110, 2nd ed., rev. and expanded., New York, New York State, 2003

Surfactant science series, 110, Second edition, revised and expanded, New York, cop. 2003

United States, United States of America

CRC Press (Unlimited), New York, 2003

2 edition, January 1, 2003

Kingdwarf -- to 2009-09

lg172435

{"edition":"2","isbns":["0824708636","9780824708634"],"last_page":928,"publisher":"CRC Press","series":"Surfactant Science"}

Includes bibliographical references and index.

Part I. Concepts -- 1. Macromolecular Adsorption: A Brief Introduction / Martinus A. Cohen Stuart 1 -- 2. Driving Forces For Protein Adsorption At Solid Surfaces / Willem Norde 21 -- 3. Thermodynamics Of Adsorption Of Amino Acids, Small Peptides, And Nucleic Acid Components On Silica Adsorbents / Vladimir A. Basiuk 45 -- 4. Quantitative Modeling Of Protein Adsorption / Charles M. Roth, Abraham M. Lenhoff 71 -- 5. Interfacial Behavior Of Protein Mutants And Variants / Martin Malmsten, Thomas Arnebrant, Peter Billsten 95 -- 6. Orientation And Activity Of Immobilized Antibodies / James N. Herron, Hsu-kun Wang, Vera Janatova, Jacob D. Durtschi, Karin Caldwell, Douglas A. Christensen, I-nan Chang, Shao-chie Huang 115 -- 7. Interactions Between Surfaces Coated With Carbohydrates, Glycolipids, And Glycoproteins / Per M. Claesson 165 -- 8. Protein Adsorption Kinetics / Jeremy J. Ramsden 199 -- 9. Mobility Of Biomolecules At Interfaces / Robert D. Tilton 221 -- 10. Protein Adsorption In Relation To Solution Association And Aggregation / Tommy Nylander 259 -- 11. Mechanism Of Interfacial Exchange Phenomena For Proteins Adsorbed At Solid-liquid Interfaces / Vincent Ball, Pierre Schaaf, Jean-claude Voegel 295 -- 12. Interactions Between Proteins And Surfactants At Solid Interfaces / Marie Wahlgren, Camilla A.-c. Karlsson, Stefan Welin-klintstrom 321 -- 13. Toward Functionalized Polyelectrolyte Biofilms / Pierre Schaaf, Jean-claude Voegel 345 -- 14. Self-assembly Of Functional Protein Multilayers: From Planar Films To Microtemplate Encapsulation / Katsuhiko Ariga, Yuri M. Lvov 367 -- 15. Biological Activity Of Adsorbed Proteins / Thomas A. Horbett 393 -- 16. Protein Interactions With Monolayers At The Air-water Interface / David W. Britt, G. Jogikalmath, Vladimir Hlady 415 -- Part Ii. Methods -- 17. Local And Global Optical Spectroscopic Probes Of Adsorbed Proteins / Vladimir Hlady, Jos Buijs 435 -- 18. Proteins On Surfaces: Methodologies For Surface Preparation And Engineering Protein Function / Krishnan K. Chittur 467 -- 19. Studies On The Conformation Of Adsorbed Proteins With The Use Of Nanoparticle Technology / Peter Billsten, Uno Carlsson, Hans Elwing 497 -- 20. Scanning Calorimetry In Probing The Structural Stability Of Proteins At Interfaces / Karin Fromell, Shao-chie Huang, Karin Caldwell 517 -- 21. Ellipsometry And Reflectometry For Studying Protein Adsorption / Martin Malmsten 539 -- 22. Surface Plasmon Resonance Spectroscopies For Protein Binding Studies At Functionalized Surfaces / Eva-kathrin Sinner, Kazutoshi Kobayashi, Thomas Lehmann, Thomas Neumann, Birgit Prein, Jurgen Ruhe, Fang Yu, Wolfgang Knoll 583 -- 23. Neutron Reflection Study Of Protein Adsorption At The Solid-solution Interface / J.r. Lu 609 -- 24. Xps, Tof-sims, And Maldi-ms For Characterizing Adsorbed Protein Films / Hans J. Griesser, Sally L. Mcarthur, Matthew S. Wagner, David G. Castner, Peter Kingshott, Keith M. Mclean 641 -- Part Iii. Applications -- 25. Interaction Of Proteins With Polymeric Synthetic Membranes / Georges Belfort, Andrew L. Zydney 671 -- 26. Protein Adsorption In Intravenous Drug Delivery / Martin Malmsten 711 -- 27. Control Of Protein Adsorption In Solid-phase Diagnostics And Therapeutics / Krister Holmberg, Gerard Quash 741 -- 28. Protein Interfacial Behavior In Microfabricated Analysis Systems And Microarrays / Helene Derand, Martin Malmsten 773 -- 29. Protein Adsorption In The Oral Environment / Thomas Arnebrant 811 -- 30. Adsorbed Biopolymers: Behavior In Food Applications / David S. Horne, J.m. Rodriguez Patino 857. Includes Bibliographical References And Index.

This new edition features research from nearly 60 of the profession's most distinguished international authorities. Recognizing emerging developments in biopolymer systems research with fully updated and expanded chapters, the second edition discusses the biopolymer-based multilayer structures and their application in biosensors, the progress made in the understanding of protein behaviour at the air-water interface, experimental findings in ellipsometry and reflectometry, and recent developments concerning protein interfacial behaviour in microfabricated total analysis systems and microarrays. With over 3000 references, this is an essential reference for professionals and students in surface, pharmaceutical, colloid, polymer, and medicinal chemistry; chemical, formulation, and application engineering; and pharmacy.

Recognizing emerging developments in biopolymer systems research, this second edition of a survey of recent work relating to the interfacial behavior of biopolymers covers concepts, methods, and applications. The book discusses biopolymer-based multilayer structures and their application in biosensors, protein behavior at the air-water interface, experimental findings in ellipsometry and reflectometry, protein interfacial behavior in microfabricated total analysis systems and microarrays, and surface plasmon resonance spectroscopies for protein binding studies at functionalized surfaces. Malmsten is managing director of the Institute for Surface Chemistry. Annotation (c)2003 Book News, Inc., Portland, OR (booknews.com)

2010-01-07